From the test tube to the cell: exploring the folding and aggregation of a beta-clam protein.

From the test tube to the cell: exploring the folding and aggregation of a beta-clam protein. Research Abstract Details 

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From the test tube to the cell: exploring the folding and aggregation of a beta-clam protein. Abstract Text:

Zoya Ignatova,Beena Krishnan,Jeffrey P Bombardier,Anna Marie C Marcelino,Jiang Hong,Lila M Gierasch,

A crucial challenge in present biomedical research is the elucidation of how fundamental processes like protein folding and aggregation occur in the complex environment of the cell. Many new physico-chemical factors like crowding and confinement must be considered, and immense technical hurdles must be overcome in order to explore these processes in vivo. Understanding protein misfolding and aggregation diseases and developing therapeutic strategies to these diseases demand that we gain mechanistic insight into behaviors and misbehaviors of proteins as they fold in vivo. We have developed a fluorescence approach using FlAsH labeling to study the thermodynamics of folding of a model beta-rich protein, cellular retinoic acid binding protein (CRABP) in Escherichia coli cells. The labeling approach has also enabled us to follow aggregation of a modified version of CRABP and chimeras between CRABP and huntingtin exon 1 with its glutamine repeat tract. In this article, we review our recent results using FlAsH labeling to study in-vivo folding and present new observations that hint at fundamental differences between the thermodynamics and kinetics of protein folding in vivo and in vitro.

From the test tube to the cell: exploring the folding and aggregation of a beta-clam protein. Publishing Authors By Initials

Z Ignatova,B Krishnan,JP Bombardier,AM Marcelino,J Hong,LM Gierasch,

For similar proteins: recombinant proteins research abstracts see: proteins: recombinant proteins research

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From the test tube to the cell: exploring the folding and aggregation of a beta-clam protein. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biopolymers

VOLUME: 88

Page Numbers: 157-63

Journal Abbreviation:

ISSN: 0006-3525

DAY: 3

MONTH: 12

YEAR: 2007

From the test tube to the cell: exploring the folding and aggregation of a beta-clam protein. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 372525

From the test tube to the cell: exploring the folding and aggregation of a beta-clam protein. Keywords Mesh Terms:

KEYWORDS: Recombinant Proteins

MESH TERMS: genetics

Chemical & Substance for Abstract: From the test tube to the cell: exploring the folding and aggregation of a beta-clam protein. Information

Substance Name: retinoic acid binding protein I, cellula

Registry Number: 0

Grant and Affiliation Information for From the test tube to the cell: exploring the folding and aggregation of a beta-clam protein.

AFFILIATION: Max Planck Institute for Biochemistry, Martinsreid, Germany.

Country: United States

AGENCY: United States NIGMS

GRANT: GM027616

ACRONYM: GM

MEDLINETA: Biopolymers

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