Frictional drag and electrical manipulation of recombinant proteins in polymer-supported membranes.

Frictional drag and electrical manipulation of recombinant proteins in polymer-supported membranes. Research Abstract Details 

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Frictional drag and electrical manipulation of recombinant proteins in polymer-supported membranes. Abstract Text:

Motomu Tanaka,Joachim Hermann,Ilka Haase,Markus Fischer,Steven G Boxer,

We establish a lipid monolayer supported by a polymer interface that offers advantages over conventional solid-supported membranes for determining the frictional drag at the membrane-protein interface as well as for electric field manipulation of membrane-anchored proteins. Polymer-supported monolayers with functional lipid anchors allow for the specific docking of His-tagged green fluorescent protein variants (His-EGFP and His-DsRed tetramer) onto the membrane surface at a defined surface density. In the first part, we measure the lateral diffusion coefficients of lipids and proteins and calculate the frictional drag at the protein-membrane interface. The second part deals with the electric field-induced accumulation of recombinant proteins on a patterned surface. The mean drift velocity of proteins, which can be obtained analytically from the shape of the steady-state concentration gradient, can be controlled by tuning the interplay of electrophoresis and electroosmosis. The results demonstrate the potential of such molecular constructs for the local functionalization of solid substrates with membrane-associated proteins.

Frictional drag and electrical manipulation of recombinant proteins in polymer-supported membranes. Publishing Authors By Initials

M Tanaka,J Hermann,I Haase,M Fischer,SG Boxer,

For similar proteins: recombinant proteins: recombinant fusion proteins research abstracts see: proteins: recombinant proteins: recombinant fusion proteins research

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Frictional drag and electrical manipulation of recombinant proteins in polymer-supported membranes. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Langmuir : the ACS journal of surfaces and colloid

VOLUME: 23

Page Numbers: 5638-44

Journal Abbreviation:

ISSN: 0743-7463

DAY: 5

MONTH: 04

YEAR: 2007

Frictional drag and electrical manipulation of recombinant proteins in polymer-supported membranes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9882736

Frictional drag and electrical manipulation of recombinant proteins in polymer-supported membranes. Keywords Mesh Terms:

KEYWORDS: Recombinant Fusion Proteins

MESH TERMS: chemistry

Chemical & Substance for Abstract: Frictional drag and electrical manipulation of recombinant proteins in polymer-supported membranes. Information

Substance Name: Histidine

Registry Number: 71-00-1

Grant and Affiliation Information for Frictional drag and electrical manipulation of recombinant proteins in polymer-supported membranes.

AFFILIATION: Department of Physics, Technical University of Munich, D-85748 Garching, Germany. tanaka@uni-heidelberg.de

Country: United States

AGENCY: United States NIGMS

GRANT: GM069630

ACRONYM: GM

MEDLINETA: Langmuir

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