Frequent activation of AKT2 kinase in human pancreatic carcinomas.

Frequent activation of AKT2 kinase in human pancreatic carcinomas. Research Abstract Details 

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Frequent activation of AKT2 kinase in human pancreatic carcinomas. Abstract Text:

Deborah A Altomare,Satoshi Tanno,Assunta De Rienzo,Andres J Klein-Szanto,Sachie Tanno,Kristine L Skele,John P Hoffman,Joseph R Testa,

Activation of AKT/protein kinase B promotes a variety of biological activities important in tumorigenesis, such as cell survival and cell cycle progression. We previously demonstrated amplification and overexpression of the AKT2 gene in a subset of human pancreatic carcinomas. In this investigation, we assessed AKT2 catalytic activity in 50 frozen pancreatic tissues (37 carcinomas, four benign tumors and nine normal pancreata) by in vitro kinase assay. Twelve of 37 (32%) pancreatic carcinomas showed markedly elevated levels of AKT2 activity compared to normal pancreata and begin pancreatic tumors. To delineate mechanisms contributing to AKT2 activation in malignant pancreatic tumors, we examined the status of upstream components of the phosphatilydlinositol 3-kinase (PI3K)/AKT pathway. Western blot analysis revealed loss of PTEN protein expression in two of the 12 pancreatic carcinomas with activated AKT2. In vitro PI3K assays demonstrated high levels of PI3K activity in seven carcinoma specimens that showed AKT2 activation. Immunohistochemical staining confirmed high levels of phosphorylated (active) AKT in malignant pancreatic tumors compared to normal pancreata. Overall, these data suggest that upstream perturbations of the PI3K/AKT pathway contribute to frequent activation of AKT2 in pancreatic cancer, which may contribute to the pathogenesis of this highly aggressive form of human malignancy.

Frequent activation of AKT2 kinase in human pancreatic carcinomas. Publishing Authors By Initials

DA Altomare,S Tanno,A De Rienzo,AJ Klein-Szanto,S Tanno,KL Skele,JP Hoffman,JR Testa,

For similar proteins: neoplasm proteins: tumor suppressor proteins research abstracts see: proteins: neoplasm proteins: tumor suppressor proteins research

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Frequent activation of AKT2 kinase in human pancreatic carcinomas. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of cellular biochemistry

VOLUME: 87

Page Numbers: 470-6

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ISSN: 0730-2312

DAY: 31

MONTH: 03

YEAR: 2002

Frequent activation of AKT2 kinase in human pancreatic carcinomas. Information

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LANGUAGE: eng

NlmUniqueID: 8205768

Frequent activation of AKT2 kinase in human pancreatic carcinomas. Keywords Mesh Terms:

KEYWORDS: Tumor Suppressor Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Frequent activation of AKT2 kinase in human pancreatic carcinomas. Information

Substance Name: PTEN Phosphohydrolase

Registry Number: EC 3.1.3.67

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Country: United States

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MEDLINETA: J Cell Biochem

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