Free-flow electrophoresis for purification of plant mitochondria by surface charge.

Free-flow electrophoresis for purification of plant mitochondria by surface charge. Research Abstract Details 

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Free-flow electrophoresis for purification of plant mitochondria by surface charge. Abstract Text:

Holger Eubel,Chun Pong Lee,John Kuo,Etienne H Meyer,Nicolas L Taylor,A Harvey Millar,Holger Eubel,Chun Pong Lee,John Kuo,Etienne H Meyer,Nicolas L Taylor,A Harvey Millar,

Sample purity is the key for a successful in-depth analysis of any given subcellular proteome. The suitability of free-flow electrophoresis to assist conventional, centrifugation-based techniques in the preparation of plant mitochondria from green and non-green tissue was assessed by various means, including functional assays, immunoblots, electron microscopy and differential gel electrophoresis. Results indicated a significant increase in purity of the mitochondrial samples, highlighted specific contaminants previously reported as mitochondrial proteins, and also pointed to new means for separating plastids and peroxisomes from mitochondria in plant organellar extracts by exploiting differences in surface charge. This approach has the potential to allow a deeper and more comprehensive investigation of the Arabidopsis organellar proteomes, by providing a second dimension of separation based on surface charge in addition to conventional centrifugation purification protocols relying on size and density.

Free-flow electrophoresis for purification of plant mitochondria by surface charge. Publishing Authors By Initials

H Eubel,CP Lee,J Kuo,EH Meyer,NL Taylor,AH Millar,H Eubel,CP Lee,J Kuo,EH Meyer,NL Taylor,AH Millar,

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Free-flow electrophoresis for purification of plant mitochondria by surface charge. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Plant journal : for cell and molecular biology

VOLUME: 52

Page Numbers: 583-94

Journal Abbreviation: Plant J.

ISSN: 0960-7412

DAY: 28

MONTH: 08

YEAR: 2007

Free-flow electrophoresis for purification of plant mitochondria by surface charge. Information

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LANGUAGE: eng

NlmUniqueID: 9207397

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Grant and Affiliation Information for Free-flow electrophoresis for purification of plant mitochondria by surface charge.

AFFILIATION: ARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, 35 Stirling Highway, M316, Crawley, WA 6009, Australia.

Country: England

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MEDLINETA: Plant J

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