Fragmentation of ceruloplasmin following non-enzymatic glycation reaction.

Fragmentation of ceruloplasmin following non-enzymatic glycation reaction. Research Abstract Details 

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Fragmentation of ceruloplasmin following non-enzymatic glycation reaction. Abstract Text:

K N Islam,M Takahashi,S Higashiyama,T Myint,N Uozumi,

Bovine ceruloplasmin underwent fragmentation following non-enzymatic glycosylation. Western blot and ELISA analyses indicated that a polyclonal rabbit antiserum to hexitolysine reacted with bovine ceruloplasmin after incubation with 0.1 M glucose. The same fragmentation was seen upon exposure of the protein to a hydrogen peroxide bolus. Both catalase and EDTA blocked peroxide-dependent fragmentation. Incubation with glucose resulted in a time-dependent release of Cu2+. The released Cu2+ appeared to participate in a Fenton-type reaction to produce hydroxyl radicals, which effected the fragmentation. Hydroxyl radical scavengers such as thiourea, mannitol, methionine, and formate inhibited this cleavage. ESR spectral studies also supported participation of hydroxyl radicals. Inhibition by EDTA of the fragmentation induced by an H2O2 bolus also supports a role for copper in a Fenton-type reaction. Taken together these results suggest that reactive oxygen species, such as superoxide anion and H2O2, were formed by the Maillard reaction which led to hydroxyl radicals being produced by a copper-dependent Fenton-type reaction. Both processes are likely to be involved in the fragmentation of ceruloplasmin.

Fragmentation of ceruloplasmin following non-enzymatic glycation reaction. Publishing Authors By Initials

KN Islam,M Takahashi,S Higashiyama,T Myint,N Uozumi,

For similar organic chemicals: alcohols: sugar alcohols: sorbitol research abstracts see: organic chemicals: alcohols: sugar alcohols: sorbitol research

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Fragmentation of ceruloplasmin following non-enzymatic glycation reaction. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 118

Page Numbers: 1054-60

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1995

Fragmentation of ceruloplasmin following non-enzymatic glycation reaction. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Fragmentation of ceruloplasmin following non-enzymatic glycation reaction. Keywords Mesh Terms:

KEYWORDS: Sorbitol

MESH TERMS: chemistry

Chemical & Substance for Abstract: Fragmentation of ceruloplasmin following non-enzymatic glycation reaction. Information

Substance Name: Ceruloplasmin

Registry Number: EC 1.16.3.1

Grant and Affiliation Information for Fragmentation of ceruloplasmin following non-enzymatic glycation reaction.

AFFILIATION: Department of Biochemistry, Osaka University Medical School.

Country: JAPAN

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MEDLINETA: J Biochem

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