FoxD3 and Grg4 physically interact to repress transcription and induce mesoderm in Xenopus.

FoxD3 and Grg4 physically interact to repress transcription and induce mesoderm in Xenopus. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

FoxD3 and Grg4 physically interact to repress transcription and induce mesoderm in Xenopus. Abstract Text:

Sergey Yaklichkin,Aaron B Steiner,Qun Lu,Daniel S Kessler,

FoxD3 is a forkhead-related transcriptional regulator that is essential for multiple developmental processes in the vertebrate embryo, including neural crest development and maintenance of mammalian stem cell lineages. Recent results demonstrate a requirement for FoxD3 in Xenopus mesodermal development. In the gastrula, FoxD3 functions as a transcriptional repressor in the Spemann organizer to maintain the expression of Nodal-related members of the transforming growth factor-beta superfamily that induce dorsal mesoderm formation. Here we report that the function of FoxD3 in mesoderm induction is dependent on the recruitment of transcriptional corepressors of the TLE/Groucho family. Structure-function analyses indicate that the transcriptional repression and mesoderm induction activities of FoxD3 are dependent on a C-terminal domain, as well as specific DNA-binding activity conferred by the forkhead domain. The C-terminal domain contains a heptapeptide similar to the eh1/GEH Groucho interaction motif. Deletion and point mutagenesis demonstrated that the FoxD3 eh1/GEH motif is required for both repression of transcription and induction of mesoderm, as well as the direct physical interaction of FoxD3 and Grg4 (Groucho-related gene-4). Consistent with a functional interaction of FoxD3 and Grg4, the transcriptional repression activity of FoxD3 is enhanced by Grg4, and reduced by Grg5, a dominant inhibitory Groucho protein. The results indicate that FoxD3 recruitment of Groucho corepressors is essential for the transcriptional repression of target genes and induction of mesoderm in Xenopus.

FoxD3 and Grg4 physically interact to repress transcription and induce mesoderm in Xenopus. Publishing Authors By Initials

S Yaklichkin,AB Steiner,Q Lu,DS Kessler,

For similar proteins: amphibian proteins: xenopus proteins research abstracts see: proteins: amphibian proteins: xenopus proteins research

PUBMED ID PMID:

MEDLINE DATE:

FoxD3 and Grg4 physically interact to repress transcription and induce mesoderm in Xenopus. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 2548-57

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 30

MONTH: 11

YEAR: 2006

FoxD3 and Grg4 physically interact to repress transcription and induce mesoderm in Xenopus. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

FoxD3 and Grg4 physically interact to repress transcription and induce mesoderm in Xenopus. Keywords Mesh Terms:

KEYWORDS: Xenopus Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: FoxD3 and Grg4 physically interact to repress transcription and induce mesoderm in Xenopus. Information

Substance Name: foxd3b-A protein, Xenopus

Registry Number: 0

Grant and Affiliation Information for FoxD3 and Grg4 physically interact to repress transcription and induce mesoderm in Xenopus.

AFFILIATION: Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM064768-04

ACRONYM: GM

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

FoxD3 and Grg4 physically interact to repress transcription and induce mesoderm in Xenopus Related Publications

• About the suboccipital part of the vertebral artery and the neighboring bone-joint and nerve relationships.
• Arthritis and physical-mental comorbidity in metropolitan China.
• Broadening the definition of generalized anxiety disorder: effects on prevalence and associations with other disorders in the National Comorbidity Survey Replication.
• Changes in surgical therapy for Crohn's disease over 33 years: a prospective longitudinal study.
• Clinical differences among patients treated for mental health problems in general medical and specialty mental health settings in the National Comorbidity Survey Replication.
• Complete robotic-assistance during laparoscopic living donor nephrectomies: an evaluation of 38 procedures at a single site.
• Concordance of the Composite International Diagnostic Interview Version 3.0 (CIDI 3.0) with standardized clinical assessments in the WHO World Mental Health surveys.
• DSM-IV personality disorders in the National Comorbidity Survey Replication.
• Delay in initial treatment contact after first onset of mental disorders in metropolitan China.
• Developmental changes in progenitor cell responsiveness to bone morphogenetic proteins differentially modulate progressive CNS lineage fate.
• Developmental changes in progenitor cell responsiveness to cytokines.
• Dual function of Sox1 in telencephalic progenitor cells.
• FoxD3 and Grg4 physically interact to repress transcription and induce mesoderm in Xenopus.
• FoxD3 regulation of Nodal in the Spemann organizer is essential for Xenopus dorsal mesoderm development.
• Lifetime prevalence and inter-cohort variation in DSM-IV disorders in metropolitan China.
• Lifetime prevalence, correlates, and persistence of oppositional defiant disorder: results from the National Comorbidity Survey Replication.
• Mental disorders among English-speaking Mexican immigrants to the US compared to a national sample of Mexicans.
• Mental health service use among hurricane Katrina survivors in the eight months after the disaster.
• Multiple congenital coagulopathies co-expressed with Von Willebrand's disease: the experience of Hemophilia Region III Treatment Centers over 25 years and review of the literature.
• Pharmacology of ampakine modulators: from AMPA receptors to synapses and behavior.
• Postcircumcision necrosis of the glans penis: gray-scale and color Doppler sonographic findings.
• Prevalence of the EH1 Groucho interaction motif in the metazoan Fox family of transcriptional regulators.
• Psychiatric epidemiology: challenges and opportunities.
• Risk for psychiatric disorder among immigrants and their US-born descendants: evidence from the National Comorbidity Survey Replication.
• Sonic hedgehog and BMP2 exert opposing actions on proliferation and differentiation of embryonic neural progenitor cells.
• Telephone screening, outreach, and care management for depressed workers and impact on clinical and work productivity outcomes: a randomized controlled trial.
• The costs and benefits of enhanced depression care to employers.
• The impact of comorbidity of mental and physical conditions on role disability in the US adult household population.
• The prevalence and correlates of eating disorders in the National Comorbidity Survey Replication.
• Understanding mental health treatment in persons without mental diagnoses: results from the National Comorbidity Survey Replication.