Four novel suppressors of gic1 gic2 and their roles in cytokinesis and polarized cell growth in Saccharomyces cerevisiae.

Four novel suppressors of gic1 gic2 and their roles in cytokinesis and polarized cell growth in Saccharomyces cerevisiae. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Four novel suppressors of gic1 gic2 and their roles in cytokinesis and polarized cell growth in Saccharomyces cerevisiae. Abstract Text:

Meghal Gandhi,Bruce L Goode,Clarence S M Chan,

Gic1 and Gic2 are two Cdc42/Rac interactive binding (CRIB) domain-containing effectors of Cdc42-GTPase that promote polarized cell growth in S. cerevisiae. To identify novel genes that functionally interact with Gic1 and Gic2, we screened for high-copy suppressors of a gic1 gic2 temperature-sensitive strain. We identified two pairs of structurally related genes, SKG6-TOS2 and VHS2-MLF3. These genes have been implicated in polarized cell growth, but their functions have not previously been characterized. We found that overproduction of Skg6 and Tos2 in wild-type cells causes aberrant localization of Cdc3 septin and actin structures as well as defective recruitment of Hof1 and impaired formation of the septum at the mother-bud neck. These data suggest a negative regulatory function for Skg6 and Tos2 in cytokinesis. Consistent with this model, deletion of SKG6 suppresses the growth defects associated with loss of HOF1, a positive regulator of cytokinesis. Our analysis of the second pair of gic1 gic2 suppressors, VHS2 and MLF3, suggests that they regulate polarization of the actin cytoskeleton and cell growth and function in a pathway distinct from and parallel to GIC1 and GIC2.

Four novel suppressors of gic1 gic2 and their roles in cytokinesis and polarized cell growth in Saccharomyces cerevisiae. Publishing Authors By Initials

M Gandhi,BL Goode,CS Chan,

For similar biological phenomena, cell phenomena, and immunity: cell physiology: cell communication: signal transduction research abstracts see: biological phenomena, cell phenomena, and immunity: cell physiology: cell communication: signal transduction research

PUBMED ID PMID:

MEDLINE DATE:

Four novel suppressors of gic1 gic2 and their roles in cytokinesis and polarized cell growth in Saccharomyces cerevisiae. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Genetics

VOLUME: 174

Page Numbers: 665-78

Journal Abbreviation: Genetics

ISSN: 0016-6731

DAY: 2

MONTH: 07

YEAR: 2006

Four novel suppressors of gic1 gic2 and their roles in cytokinesis and polarized cell growth in Saccharomyces cerevisiae. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 374636

Four novel suppressors of gic1 gic2 and their roles in cytokinesis and polarized cell growth in Saccharomyces cerevisiae. Keywords Mesh Terms:

KEYWORDS: Signal Transduction

MESH TERMS: genetics

Chemical & Substance for Abstract: Four novel suppressors of gic1 gic2 and their roles in cytokinesis and polarized cell growth in Saccharomyces cerevisiae. Information

Substance Name: Skg6 protein, S cerevisiae

Registry Number: 0

Grant and Affiliation Information for Four novel suppressors of gic1 gic2 and their roles in cytokinesis and polarized cell growth in Saccharomyces cerevisiae.

AFFILIATION: Section of Molecular Genetics and Microbiology, University of Texas, Texas 78712, USA. meghal@brandeis.edu

Country: United States

AGENCY: United States NIGMS

GRANT: GM63691

ACRONYM: GM

MEDLINETA: Genetics

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Four novel suppressors of gic1 gic2 and their roles in cytokinesis and polarized cell growth in Saccharomyces cerevisiae Related Publications

• A truncated Ah receptor blocks the hypoxia and estrogen receptor signaling pathways: a viable approach for breast cancer treatment.
• Activation of the Akt/GSK3beta signaling pathway mediates survival of vulnerable hippocampal neurons after transient global cerebral ischemia in rats.
• Anaplastic large cell Ki-1 lymphoma. Delineation of two morphological types. J. K. C. Chan, C. S. Ng, P. K. Hui, T. W. Leung, E. S. F. Lo, W. H. Lau, L. J. McGuire. Histopathology 1989; 15; 11-34.
• Antibody detection using tumor-associated antigen mini-array in immunodiagnosing human hepatocellular carcinoma.
• Computer-aided detection system for clustered microcalcifications: comparison of performance on full-field digital mammograms and digitized screen-film mammograms.
• Dural metastases mimicking meningioma. Case report and review of the literature.
• Dynamics of smooth muscle cell deadhesion from thermosensitive hydroxybutyl chitosan.
• Effectiveness of brachytherapy and fractionated stereotactic radiotherapy boost for persistent nasopharyngeal carcinoma.
• Evaluating measurement error in estimates of worker exposure assessed in parallel by personal and biological monitoring.
• Evaluation of a fluorescent method for measuring cholinesterase activity in mammalian blood and tissue.
• Extrapancreatic proinsulin/insulin-expressing cells in diabetes mellitus: is history repeating itself?
• Four novel suppressors of gic1 gic2 and their roles in cytokinesis and polarized cell growth in Saccharomyces cerevisiae.
• Gastrointestinal carcinoids: the evolution of diagnostic strategies.
• Genetics and Parkinson's disease.
• Ikaros is required for plasmacytoid dendritic cell differentiation.
• Influence of premorbid IQ and education on progression of Alzheimer's disease.
• Is fundamental frequency a cue to aspiration in initial stops?
• Magnesium sulphate in aneurysmal subarachnoid haemorrhage - the need for a large multi-center study.
• Mitochondrial fusion and fission in mammals.
• Mitochondrial translocation of p53 underlies the selective death of hippocampal CA1 neurons after global cerebral ischaemia.
• Molecular cloning and functional study of rat estrogen receptor-related receptor gamma in rat prostatic cells.
• Physiological factors of the small intestine in design of active capsule endoscopy.
• Pre-operative parenteral nutrition in patients with oesophageal cancer: a prospective, randomised clinical trial.
• The N-terminal transmembrane domain (TMD0) and a cytosolic linker (L0) of sulphonylurea receptor define the unique intrinsic gating of KATP channels.
• The effects of filling techniques and a low-viscosity composite liner on bond strength to class II cavities.
• The relationship of ambulatory blood pressure to physical activity in a tri-ethnic population of obese and nonobese adolescents.
• Vitamin E in renal therapeutic regimens.
• [Distributings and changes of NO and calcitonin gene-related peptide in pharyngeal tissues and plasma in obstructive sleep apnea and hypopnea syndrome]
• [Study on the identification of dangguitou and dangguiwei by two-dimensional infrared correlation spectroscopy]
• [Treatment of 113 oral and maxillofacial hemangiomas with long pulsed turnable 1064 nm Gentle YAG laser]