Formulation and in-vitro and in-vivo evaluation of a mucoadhesive gel containing freeze dried black raspberries: implications for oral cancer chemoprevention.

Formulation and in-vitro and in-vivo evaluation of a mucoadhesive gel containing freeze dried black raspberries: implications for oral cancer chemoprevention. Research Abstract Details 

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Formulation and in-vitro and in-vivo evaluation of a mucoadhesive gel containing freeze dried black raspberries: implications for oral cancer chemoprevention. Abstract Text:

Susan R Mallery,Gary D Stoner,Peter E Larsen,Henry W Fields,Kapila A Rodrigo,Steven J Schwartz,Qingguo Tian,Jin Dai,Russell J Mumper,

PURPOSE: The purpose of these studies was to formulate mucoadhesive gels containing freeze dried black raspberries (FBR) and to determine optimum parameters for a subset of FBR bioactive compounds including anthocyanin stability, absorption and penetration in-vitro and in-vivo. MATERIALS AND METHODS: Berry gels were prepared having FBR at 5% and 10% w/w and final pHs ranging from 3.5 to 7.5. A HPLC assay was developed to quantify and determine the stability of the anthocyanins in the gels. A single time-point study was performed to determine anthocyanin uptake when the gels were applied to oral mucosa. Penetration of anthocyanins into human oral tissue explants was determined as a function of gel pH and FBR content. A HPLC-mass spectroscopy assay was utilized to quantify the anthocyanin levels in human oral tissue explants, saliva, and blood. RESULTS: The stability of anthocyanins in the gel was directly related to gel pH and storage temperature. Maximum stability of anthocyanins was found at lower pH (pH 3.5) and storage temperature (4 degrees C). Anthocyanins contained in mucoadhesive berry gel formulations were readily absorbed into human oral mucosa tissue as evidenced by detectable blood levels within 5 min after gel application. There was a trend for greater penetration of anthocyanins into tissue explants for berry gels with a final pH of 6.5 versus pH 3.5. CONCLUSIONS: Formulation and characterization of a novel gel formulation for local delivery of chemopreventive compounds to human oral mucosal tissues has been described. The results show anthocyanin stability was dependent upon gel pH and storage temperature and also demonstrate that the gel composition is well-suited for absorption and penetration into the target oral mucosal tissue site.

Formulation and in-vitro and in-vivo evaluation of a mucoadhesive gel containing freeze dried black raspberries: implications for oral cancer chemoprevention. Publishing Authors By Initials

SR Mallery,GD Stoner,PE Larsen,HW Fields,KA Rodrigo,SJ Schwartz,Q Tian,J Dai,RJ Mumper,

For similar investigative techniques: clinical laboratory techniques: culture techniques: tissue culture techniques research abstracts see: investigative techniques: clinical laboratory techniques: culture techniques: tissue culture techniques research

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Formulation and in-vitro and in-vivo evaluation of a mucoadhesive gel containing freeze dried black raspberries: implications for oral cancer chemoprevention. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Pharmaceutical research

VOLUME: 24

Page Numbers: 728-37

Journal Abbreviation: Pharm. Res.

ISSN: 0724-8741

DAY: 15

MONTH: 02

YEAR: 2007

Formulation and in-vitro and in-vivo evaluation of a mucoadhesive gel containing freeze dried black raspberries: implications for oral cancer chemoprevention. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8406521

Formulation and in-vitro and in-vivo evaluation of a mucoadhesive gel containing freeze dried black raspberries: implications for oral cancer chemoprevention. Keywords Mesh Terms:

KEYWORDS: Tissue Culture Techniques

MESH TERMS: metabolism

Chemical & Substance for Abstract: Formulation and in-vitro and in-vivo evaluation of a mucoadhesive gel containing freeze dried black raspberries: implications for oral cancer chemoprevention. Information

Substance Name: Plant Extracts

Registry Number: 0

Grant and Affiliation Information for Formulation and in-vitro and in-vivo evaluation of a mucoadhesive gel containing freeze dried black raspberries: implications for oral cancer chemoprevention.

AFFILIATION: Department of Oral Maxillofacial Surgery & Pathology, College of Dentistry, The Ohio State University, Columbus, OH 43210-1241, USA. mallery.1@osu.edu

Country: United States

AGENCY: United States NCI

GRANT: R21 CA11120

ACRONYM: CA

MEDLINETA: Pharm Res

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