Formate dehydrogenase. Subunit and mechanism of inhibition by cyanide and azide.

Formate dehydrogenase. Subunit and mechanism of inhibition by cyanide and azide. Research Abstract Details 

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Formate dehydrogenase. Subunit and mechanism of inhibition by cyanide and azide. Abstract Text:

T Ohyama,I Yamazaki,

Formate dehydrogenase (EC 1.2.1.2) prepared from peas (Pisum sativum) was a two-subunit enzyme. The enzyme accelerated the formation of an NAD+-cyanide compound having an adsorption band at 330 nm. The enzyme was able to bind one NAD+ molecule per each subunit but only 1 mole of NAD+-cyanide compound was formed per two subunits. The complex of NAD+, cyanide, and the enzyme was very stable and had no catalytic activity. Azide inhibited the formate dehydrogenase reaction in two different ways. By incubation of the enzyme with azide in the presence of NAD+, half of its catalytic activity was lost. The remaining activity was also inhibited by azide but this inhibition was removed competively by formate. Contrary to the case of cyanide the inhibition by azide could be removed by dialysis and no spectral species due to the addition compound of NAD+ and azide could be observed. The data from double recipricol plots of the initial velocity and the formate concentration led to a conclusion that formate dehydrogenase has two sites with about equal catalytic activity. The Km for formate was different for the two catalytic sites (1.67 and 6.25 mM) but the difference was not noticeable in the case of the Km for NAD+.

Formate dehydrogenase. Subunit and mechanism of inhibition by cyanide and azide. Publishing Authors By Initials

T Ohyama,I Yamazaki,

For similar investigative techniques: chemistry, analytical: photometry: spectrophotometry research abstracts see: investigative techniques: chemistry, analytical: photometry: spectrophotometry research

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Formate dehydrogenase. Subunit and mechanism of inhibition by cyanide and azide. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 77

Page Numbers: 845-52

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Apr

YEAR: 1975

Formate dehydrogenase. Subunit and mechanism of inhibition by cyanide and azide. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Formate dehydrogenase. Subunit and mechanism of inhibition by cyanide and azide. Keywords Mesh Terms:

KEYWORDS: Spectrophotometry

MESH TERMS: enzymology

Chemical & Substance for Abstract: Formate dehydrogenase. Subunit and mechanism of inhibition by cyanide and azide. Information

Substance Name: Aldehyde Oxidoreductases

Registry Number: EC 1.2.-

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Country: JAPAN

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MEDLINETA: J Biochem

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