Food vacuole targeting and trafficking of falcipain-2, an important cysteine protease of human malaria parasite Plasmodium falciparum.

Food vacuole targeting and trafficking of falcipain-2, an important cysteine protease of human malaria parasite Plasmodium falciparum. Research Abstract Details 

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Food vacuole targeting and trafficking of falcipain-2, an important cysteine protease of human malaria parasite Plasmodium falciparum. Abstract Text:

Palakodeti V N Dasaradhi,Reshma Korde,Jennifer K Thompson,Charu Tanwar,Tapas C Nag,Virander S Chauhan,Alan F Cowman,Asif Mohmmed,Pawan Malhotra,

Malaria proteases are attractive anti-malarial targets because of their roles in parasite development and infection. Falcipain-2 (FP-2), a food vacuole cysteine protease in Plasmodium falciparum, is involved in hemoglobin degradation and cleavage of cytoskeletal elements. To understand the route of trafficking and identify the signals involved in trafficking to food vacuole, we have generated transgenic parasites expressing green fluorescent protein (GFP) fusion proteins comprising of N-terminal regions of falcipain-2 fused to GFP. Using falcipain2-GFP chimeras and anti-falcipain-2 antibody, we show that falcipain-2 is trafficked through a classical vesicle mediated secretory pathway involving endoplasmic reticulum and Golgi-like apparatus. Photobleaching and confocal microscopy techniques reveal that falcipain-2 is carried to the food vacuole in the form of cytostomal vesicles. We identify an N-terminal sequence (1-120aa) of falcipain-2, sufficient for its transport to the food vacuole. Analysis of sequences of few other food vacuole targeted proteins suggests a common mechanism for protein trafficking to food vacuole of malaria parasite.

Food vacuole targeting and trafficking of falcipain-2, an important cysteine protease of human malaria parasite Plasmodium falciparum. Publishing Authors By Initials

PV Dasaradhi,R Korde,JK Thompson,C Tanwar,TC Nag,VS Chauhan,AF Cowman,A Mohmmed,P Malhotra,

For similar cells: cellular structures: intracellular space: cytoplasm: cytoplasmic structures: organelles: cytoplasmic vesicles: vacuoles research abstracts see: cells: cellular structures: intracellular space: cytoplasm: cytoplasmic structures: organelles: cytoplasmic vesicles: vacuoles research

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Food vacuole targeting and trafficking of falcipain-2, an important cysteine protease of human malaria parasite Plasmodium falciparum. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular and biochemical parasitology

VOLUME: 156

Page Numbers: 12-23

Journal Abbreviation: Mol. Biochem. Parasitol.

ISSN: 0166-6851

DAY: 28

MONTH: 06

YEAR: 2007

Food vacuole targeting and trafficking of falcipain-2, an important cysteine protease of human malaria parasite Plasmodium falciparum. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8006324

Food vacuole targeting and trafficking of falcipain-2, an important cysteine protease of human malaria parasite Plasmodium falciparum. Keywords Mesh Terms:

KEYWORDS: Vacuoles

MESH TERMS: enzymology

Chemical & Substance for Abstract: Food vacuole targeting and trafficking of falcipain-2, an important cysteine protease of human malaria parasite Plasmodium falciparum. Information

Substance Name: falcipain 2

Registry Number: EC 3.4.22.-

Grant and Affiliation Information for Food vacuole targeting and trafficking of falcipain-2, an important cysteine protease of human malaria parasite Plasmodium falciparum.

AFFILIATION: International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India.

Country: Netherlands

AGENCY: United Kingdom Wellcome T

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MEDLINETA: Mol Biochem Parasitol

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