Focal adhesion disassembly is an essential early event in hepatic stellate cell chemotaxis.

Focal adhesion disassembly is an essential early event in hepatic stellate cell chemotaxis. Research Abstract Details 

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Focal adhesion disassembly is an essential early event in hepatic stellate cell chemotaxis. Abstract Text:

Andrew C Melton,Russell K Soon,J Genevieve Park,Luis Martinez,Gregory W Dehart,Hal F Yee,Andrew C Melton,Russell K Soon,J Genevieve Park,Luis Martinez,Gregory W Dehart,Hal F Yee,Andrew C Melton,Russell K Soon,J Genevieve Park,Luis Martinez,Gregory W Dehart,Hal F Yee,

Chemotaxis (i.e., directed migration) of hepatic stellate cells to areas of inflammation is a requisite event in the liver's response to injury. Previous studies of signaling pathways that regulate stellate cell migration suggest a key role for focal adhesions, but the exact function of these protein complexes in motility remains unclear. Focal adhesions attach a cell to its substrate and therefore must be regulated in a highly coordinated manner during migration. To test the hypothesis that focal adhesion turnover is an essential early event for chemotaxis in stellate cells, we employed a live-cell imaging technique in which chemotaxis was induced by locally stimulating the tips of rat stellate cell protrusions with platelet-derived growth factor-BB (PDGF). Focal adhesions were visualized with an antibody directed against vinculin, a structural component of the focal adhesion complex. PDGF triggered rapid disassembly of adhesions within 6.25 min, subsequent reassembly by 12.5 min, and continued adhesion assembly in concert with the spreading protrusion until the completion of chemotaxis. Blockade of adhesion disassembly by growing cells on fibronectin or treatment with nocodazole prevented a chemotactic response to PDGF. Augmentation of adhesion disassembly with ML-7 enhanced the chemotactic response to PDGF. These data suggest that focal adhesion disassembly is an essential early event in stellate cell chemotaxis in response to PDGF.

Focal adhesion disassembly is an essential early event in hepatic stellate cell chemotaxis. Publishing Authors By Initials

AC Melton,RK Soon,JG Park,L Martinez,GW Dehart,HF Yee,AC Melton,RK Soon,JG Park,L Martinez,GW Dehart,HF Yee,AC Melton,RK Soon,JG Park,L Martinez,GW Dehart,HF Yee,

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Focal adhesion disassembly is an essential early event in hepatic stellate cell chemotaxis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: American journal of physiology. Gastrointestinal a

VOLUME: 293

Page Numbers: G1272-80

Journal Abbreviation: Am. J. Physiol. Gastrointest.

ISSN: 0193-1857

DAY: 18

MONTH: 10

YEAR: 2007

Focal adhesion disassembly is an essential early event in hepatic stellate cell chemotaxis. Information

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LANGUAGE: eng

NlmUniqueID: 100901227

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AFFILIATION: Jr., San Francisco General Hospital, Bldg. 40, Rm. 4102, 1001 Potrero Ave., San Francisco, CA 94110. hyee@medsfgh.ucsf.edu).

Country: United States

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MEDLINETA: Am J Physiol Gastrointest Live

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