Foamy-virus-mediated gene transfer to canine repopulating cells.

Foamy-virus-mediated gene transfer to canine repopulating cells. Research Abstract Details 

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Foamy-virus-mediated gene transfer to canine repopulating cells. Abstract Text:

Hans-Peter Kiem,James Allen,Grant Trobridge,Erik Olson,Kirsten Keyser,Laura Peterson,David W Russell,

Foamy virus (FV) vectors are particularly attractive gene-transfer vectors for stem-cell gene therapy because they form a stable transduction intermediate in quiescent cells and can efficiently transduce hematopoietic stem cells. Here, we studied the use of FV vectors to transduce long-term hematopoietic repopulating cells in the dog, a clinically relevant large animal model. Mobilized canine peripheral blood (PB) CD34+ cells were transduced with an enhanced green fluorescent protein (EGFP)-expressing FV vector in an 18-hour transduction protocol. All 3 dogs studied had rapid neutrophil engraftment to greater than 500/microL with a median of 10 days. Transgene expression was detected in all cell lineages (B cells, T cells, granulocytes, red blood cells, and platelets), indicating multilineage engraftment of transduced cells. Up to 19% of blood cells were EGFP+, and this was confirmed at the DNA level by real-time polymerase chain reaction (PCR) and Southern blot analysis. These transduction rates were higher than the best results we obtained previously with lentiviral vectors in a similar transduction protocol. Integration site analysis also demonstrated polyclonal repopulation and the transduction of multipotential hematopoietic repopulating cells. These data suggest that FV vectors should be useful for stem-cell gene therapy, particularly for applications in which short transduction protocols are critical.

Foamy-virus-mediated gene transfer to canine repopulating cells. Publishing Authors By Initials

HP Kiem,J Allen,G Trobridge,E Olson,K Keyser,L Peterson,DW Russell,

For similar surgical procedures, operative: transplantation: transplantation, autologous research abstracts see: surgical procedures, operative: transplantation: transplantation, autologous research

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Foamy-virus-mediated gene transfer to canine repopulating cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Blood

VOLUME: 109

Page Numbers: 65-70

Journal Abbreviation: Blood

ISSN: 0006-4971

DAY: 12

MONTH: 09

YEAR: 2006

Foamy-virus-mediated gene transfer to canine repopulating cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7603509

Foamy-virus-mediated gene transfer to canine repopulating cells. Keywords Mesh Terms:

KEYWORDS: Transplantation, Autologous

MESH TERMS: genetics

Chemical & Substance for Abstract: Foamy-virus-mediated gene transfer to canine repopulating cells. Information

Substance Name: Phosphoglycerate Kinase

Registry Number: EC 2.7.2.3

Grant and Affiliation Information for Foamy-virus-mediated gene transfer to canine repopulating cells.

AFFILIATION: Clinical Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N D1-100, PO Box 19024, Seattle, WA 98109-1024, USA. hkiem@fhcrc.org

Country: United States

AGENCY: United States NHLBI

GRANT: HL53750

ACRONYM: HL

MEDLINETA: Blood

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