FNR is a global regulator of virulence and anaerobic metabolism in Salmonella enterica serovar Typhimurium (ATCC 14028s).

FNR is a global regulator of virulence and anaerobic metabolism in Salmonella enterica serovar Typhimurium (ATCC 14028s). Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

FNR is a global regulator of virulence and anaerobic metabolism in Salmonella enterica serovar Typhimurium (ATCC 14028s). Abstract Text:

Ryan C Fink,Matthew R Evans,Steffen Porwollik,Andres Vazquez-Torres,Jessica Jones-Carson,Bryan Troxell,Stephen J Libby,Michael McClelland,Hosni M Hassan,

Salmonella enterica serovar Typhimurium must successfully transition the broad fluctuations in oxygen concentrations encountered in the host. In Escherichia coli, FNR is one of the main regulatory proteins involved in O2 sensing. To assess the role of FNR in serovar Typhimurium, we constructed an isogenic fnr mutant in the virulent wild-type strain (ATCC 14028s) and compared their transcriptional profiles and pathogenicities in mice. Here, we report that, under anaerobic conditions, 311 genes (6.80% of the genome) are regulated directly or indirectly by FNR; of these, 87 genes (28%) are poorly characterized. Regulation by FNR in serovar Typhimurium is similar to, but distinct from, that in E. coli. Thus, genes/operons involved in aerobic metabolism, NO. detoxification, flagellar biosynthesis, motility, chemotaxis, and anaerobic carbon utilization are regulated by FNR in a fashion similar to that in E. coli. However, genes/operons existing in E. coli but regulated by FNR only in serovar Typhimurium include those coding for ethanolamine utilization, a universal stress protein, a ferritin-like protein, and a phosphotransacetylase. Interestingly, Salmonella-specific genes/operons regulated by FNR include numerous virulence genes within Salmonella pathogenicity island 1 (SPI-1), newly identified flagellar genes (mcpAC, cheV), and the virulence operon (srfABC). Furthermore, the role of FNR as a positive regulator of motility, flagellar biosynthesis, and pathogenesis was confirmed by showing that the mutant is nonmotile, lacks flagella, is attenuated in mice, and does not survive inside macrophages. The inability of the mutant to survive inside macrophages is likely due to its sensitivity to the reactive oxygen species generated by NADPH phagocyte oxidase.

FNR is a global regulator of virulence and anaerobic metabolism in Salmonella enterica serovar Typhimurium (ATCC 14028s). Publishing Authors By Initials

RC Fink,MR Evans,S Porwollik,A Vazquez-Torres,J Jones-Carson,B Troxell,SJ Libby,M McClelland,HM Hassan,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: virulence research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: virulence research

PUBMED ID PMID:

MEDLINE DATE:

FNR is a global regulator of virulence and anaerobic metabolism in Salmonella enterica serovar Typhimurium (ATCC 14028s). Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of bacteriology

VOLUME: 189

Page Numbers: 2262-73

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 12

MONTH: 01

YEAR: 2007

FNR is a global regulator of virulence and anaerobic metabolism in Salmonella enterica serovar Typhimurium (ATCC 14028s). Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985120

FNR is a global regulator of virulence and anaerobic metabolism in Salmonella enterica serovar Typhimurium (ATCC 14028s). Keywords Mesh Terms:

KEYWORDS: Virulence

MESH TERMS: metabolism

Chemical & Substance for Abstract: FNR is a global regulator of virulence and anaerobic metabolism in Salmonella enterica serovar Typhimurium (ATCC 14028s). Information

Substance Name: Transcription Factors

Registry Number: 0

Grant and Affiliation Information for FNR is a global regulator of virulence and anaerobic metabolism in Salmonella enterica serovar Typhimurium (ATCC 14028s).

AFFILIATION: Department of Microbiology, North Carolina State University, Raleigh, NC 27695-7615, USA.

Country: United States

AGENCY: United States NCRR

GRANT: RR16082

ACRONYM: RR

MEDLINETA: J Bacteriol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

FNR is a global regulator of virulence and anaerobic metabolism in Salmonella enterica serovar Typhimurium ATCC 14028s Related Publications

• A prescription for improving national health care.
• A quintuple therapy for H. pylori eradication.
• Amino acid side chain interactions in the presence of salts.
• CXCL10/IP-10: A missing link between inflammation and anti-angiogenesis in preeclampsia?
• Clinical significance of the presence of amniotic fluid 'sludge' in asymptomatic patients at high risk for spontaneous preterm delivery.
• Constituents of Persicaria stagnina.
• Culture and mental health of women in South-East Asia.
• Delayed presentation of penetrating craniocerebral injury caused by a nail.
• Development of a multiplex real-time PCR assay using SYBR Green 1 chemistry for simultaneous detection and subtyping of H9N2 influenza virus type A.
• Dietary mutagen exposure and risk of pancreatic cancer.
• Effect of immunomodulator daraprim on potentiation of vaccine protection of leishmania major in BALB/c mice.
• Effect of juvenile hormone and ecdysone on initiating blood meal--dependent vitellogenic ovarian cycles in autogenous and anautogenous Culex pipiens (Diptera : Culicidae).
• Emergency medicine in the UK: the future is bright!
• External cephalic version for breech presentation at term.
• FNR is a global regulator of virulence and anaerobic metabolism in Salmonella enterica serovar Typhimurium (ATCC 14028s).
• Letter to the editor.
• Maternal serum concentrations of the chemokine CXCL10/IP-10 are elevated in acute pyelonephritis during pregnancy.
• OC144: Clinical significance of the presence of amniotic fluid 'sludge' in patients with cervical cerclage.
• OP12.09: The value of the sagittal view of the 'ductal arch plane' in the prenatal diagnosis and counseling of conotruncal anomalies using 4D ultrasonography.
• Reverse transcriptase-polymerase chain reaction-enzyme linked immunosorbent assay for rapid detection of avian influenza virus subtype H9N2.
• Room temperature sphere-to-rod growth and gelation of PEO-PPO-PEO triblock copolymers in aqueous salt solutions.
• Stroke: Incidence and pattern in Benghazi, Libya.
• Structural variability of the carboxy-terminus of Epstein-Barr virus encoded latent membrane protein 1 gene in Hodgkin's lymphomas.
• Systemic lupus erythematosus presenting as massive ascites and pancreatitis.
• The prognostic role of donor corneoscleral rim cultures in corneal transplantation.
• The role of the sagittal view of the ductal arch in identification of fetuses with conotruncal anomalies using 4-dimensional ultrasonography.
• The simultaneous assay of emetine and cephaeline in ipecacuanha and its preparations by spectrofluorimetry.
• Transvaginal sonographic measurement of cervical length vs. Bishop score in labor induction at term: tolerability and prediction of Cesarean delivery.
• Treatment of anterolateral impingements of the ankle joint by arthroscopy.
• Ultrafiltered milk reduces bitterness in reduced-fat Cheddar cheese made with an exopolysaccharide-producing culture.