Flux profile and modularity analysis of time-dependent metabolic changes of de novo adipocyte formation.

Flux profile and modularity analysis of time-dependent metabolic changes of de novo adipocyte formation. Research Abstract Details 

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Flux profile and modularity analysis of time-dependent metabolic changes of de novo adipocyte formation. Abstract Text:

Yaguang Si,Jeongah Yoon,Kyongbum Lee,

White adipose tissue (WAT) mass is the main determinant of obesity and associated health risks. WAT expansion results from increases in white adipocyte cell number and size, which in turn reflect a series of shifts in the cellular metabolic state. To quantitatively profile the metabolic alterations occurring during de novo adipocyte formation, metabolic flux analysis (MFA) was used in conjunction with a novel modularity analysis algorithm on differentiating 3T3-L1 preadipocytes. Use of a type I collagen gel as an effective long-term culture substrate was also assessed. The calculated flux distributions predicted the sequential activation of several intracellular cross-compartmental pathways, including lipogenesis, the pentose phosphate pathway, and the malate cycle, in good agreement with earlier isotopic tracer experiments and gene profiling studies. Partition of the adipocyte metabolic network into highly interacting reaction subgroups suggested a functional reorganization of the major pathways consistent with the lipid-loading phenotype of the adipocyte. Flux and modularity analysis results together point to the flux distribution around pyruvate as a key indicator of adipocyte lipid accumulation.

Flux profile and modularity analysis of time-dependent metabolic changes of de novo adipocyte formation. Publishing Authors By Initials

Y Si,J Yoon,K Lee,

For similar genetic phenomena: phenotype research abstracts see: genetic phenomena: phenotype research

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Flux profile and modularity analysis of time-dependent metabolic changes of de novo adipocyte formation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of physiology. Endocrinology and

VOLUME: 292

Page Numbers: E1637-46

Journal Abbreviation: Am. J. Physiol. Endocrinol. Me

ISSN: 0193-1849

DAY: 6

MONTH: 02

YEAR: 2007

Flux profile and modularity analysis of time-dependent metabolic changes of de novo adipocyte formation. Information

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LANGUAGE: eng

NlmUniqueID: 100901226

Flux profile and modularity analysis of time-dependent metabolic changes of de novo adipocyte formation. Keywords Mesh Terms:

KEYWORDS: Phenotype

MESH TERMS: metabolism

Chemical & Substance for Abstract: Flux profile and modularity analysis of time-dependent metabolic changes of de novo adipocyte formation. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Flux profile and modularity analysis of time-dependent metabolic changes of de novo adipocyte formation.

AFFILIATION: Department of Biology, Tufts University, Medford, MA 02155, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: 1-R21-DK-67228-01

ACRONYM: DK

MEDLINETA: Am J Physiol Endocrinol Metab

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