Fluorometric measurement of urinary alpha-L-iduronidase activity.

Fluorometric measurement of urinary alpha-L-iduronidase activity. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Fluorometric measurement of urinary alpha-L-iduronidase activity. Abstract Text:

M Isemura,H Kosaka,T Ikenaka,R Kido,T Yoshimura,

A fluorogenic substrate for alpha-L-iduronidase, 4-methylumbelliferyl alpha-L-iduronide, has been newly synthesized and the enzyme activity has been measured in urine samples obtained from normal persons and patients suffering from mucopolysaccharidosis. Urine samples derived from a patient with Scheie syndrome showed greatly reduced activity compared with a normal adult at a similar age. This patient exhibited a high level of urinary excretion of dermatan sulfate and heparan sulfate, which could be interpreted in terms of her low alpha-L-iduronidase activity. The use of the fluorogenic substrate has some advantages over existing methods because of the high sensitivity and the relative ease of handling, and it should be useful not only for diagnosis but also for following the purification process of the enzyme.

Fluorometric measurement of urinary alpha-L-iduronidase activity. Publishing Authors By Initials

M Isemura,H Kosaka,T Ikenaka,R Kido,T Yoshimura,

For similar congenital, hereditary, and neonatal diseases and abnormalities: genetic diseases, inborn: metabolism, inborn errors: carbohydrate metabolism, inborn errors: mucopolysaccharidoses: mucopolysaccharidosis i research abstracts see: congenital, hereditary, and neonatal diseases and abnormalities: genetic diseases, inborn: metabolism, inborn errors: carbohydrate metabolism, inborn errors: mucopolysaccharidoses: mucopolysaccharidosis i research

PUBMED ID PMID:

MEDLINE DATE:

Fluorometric measurement of urinary alpha-L-iduronidase activity. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 84

Page Numbers: 627-32

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1978

Fluorometric measurement of urinary alpha-L-iduronidase activity. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Fluorometric measurement of urinary alpha-L-iduronidase activity. Keywords Mesh Terms:

KEYWORDS: Mucopolysaccharidosis I

MESH TERMS: urine

Chemical & Substance for Abstract: Fluorometric measurement of urinary alpha-L-iduronidase activity. Information

Substance Name: Iduronidase

Registry Number: EC 3.2.1.76

Grant and Affiliation Information for Fluorometric measurement of urinary alpha-L-iduronidase activity.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Fluorometric measurement of urinary alpha-L-iduronidase activity Related Publications

• A nested case-control study of stomach cancer and serum insulin-like growth factor (IGF)-1, IGF-2 and IGF-binding protein (IGFBP)-3.
• A prospective study of green tea consumption and oral cancer incidence in Japan.
• Beneficial effects of suplatast tosilate (IPD-1151T) in a rat cystitis model induced by intravesical hydrochloric acid.
• Characterization of wound drainage fluids as a source of soluble factors associated with wound healing: comparison with platelet-rich plasma and potential use in cell culture.
• Circadian clock genes and photoperiodism: Comprehensive analysis of clock gene expression in the mediobasal hypothalamus, the suprachiasmatic nucleus, and the pineal gland of Japanese Quail under various light schedules.
• Effects of IP-751, ajulemic acid, on bladder overactivity induced by bladder irritation in rats.
• Effects of anaesthesia on the nitrergic pathway during the micturition reflex in rats.
• Follow-up results of 71 patients undergoing metallic stent placement for the treatment of a malignant obstruction of the superior vena cava.
• Functional analysis of active urethral closure mechanisms under sneeze induced stress condition in a rat model of birth trauma.
• Further evidence that major outer membrane proteins homologous to OmpA in Porphyromonas gingivalis stabilize bacterial cells.
• Herpes simplex virus vector-mediated delivery of glial cell line-derived neurotrophic factor rescues erectile dysfunction following cavernous nerve injury.
• Histone deacetylase inhibitor-induced glycodelin enhances the initial step of implantation.
• Ifi202, an IFN-inducible candidate gene for lupus susceptibility in NZB/W F1 mice, is a positive regulator for NF-kappaB activation in dendritic cells.
• Inhibitory roles of peripheral nitrergic mechanisms in capsaicin-induced detrusor overactivity in the rat.
• Interobserver variation in the histopathological assessment of nonalcoholic steatohepatitis.
• Isoform-specific enhancement of adenylyl cyclase activity by n-alkanols.
• Local effects of antimuscarinics.
• Lower urinary tract symptoms (LUTS) and bladder afferent activity.
• Mortality attributable to cigarette smoking in a cohort study in Japan.
• Neurophysiology of stress urinary incontinence.
• Novel mutations in the GRK1 gene in Japanese patients With Oguchi disease.
• Odor-intensity Coding in the Anterior Piriform Cortex.
• Polypoidal choroidal vasculopathy examined with en face optical coherence tomography.
• Reduction of aluminum toxicity by 2-isopropylmalic acid in the budding yeast Saccharomyces cerevisiae.
• Retinal pigment epithelial tear in polypoidal choroidal vasculopathy.
• Rice gibberellin-insensitive dwarf mutant gene Dwarf 1 encodes the alpha-subunit of GTP-binding protein.
• Role of noradrenergic pathways in sneeze-induced urethral continence reflex in rats.
• The differential contractile responses to capsaicin and anandamide in muscle strips isolated from the rat urinary bladder.
• The effect of dendritic cells on the retinal cell transplantation.
• Torquetenovirus infection among northeastern Thai blood donors.