Fluoride-binding to the Escherichia coli bd-type ubiquinol oxidase studied by visible absorption and EPR spectroscopies.

Fluoride-binding to the Escherichia coli bd-type ubiquinol oxidase studied by visible absorption and EPR spectroscopies. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Fluoride-binding to the Escherichia coli bd-type ubiquinol oxidase studied by visible absorption and EPR spectroscopies. Abstract Text:

M Tsubaki,T Mogi,H Hori,

Cytochrome bd-type ubiquinol oxidase in the aerobic respiratory chain of Escherichia coli contains two hemes b (b558 and b595) and one heme d as redox metal centers. To clarify the structure of the reaction center, we analyzed the fully oxidized enzyme by visible and EPR spectroscopies using fluoride ion as a monitoring probe. The visible spectral changes upon fluoride-binding were typical of ferric iron-chlorine species, indicating heme d as a primary binding site. The negative peak at 645 nm in the difference spectrum indicates that heme b595 also provides the low-affinity fluoride-binding site. Fluoride-binding caused a complete disappearance from the EPR spectra of the low-spin signals ascribable to heme d and spectral changes in both rhombic and axial high-spin signals. After fluoride-binding, each component of the rhombic high-spin signal showed superhyperfine splitting arising from the interaction of the unpaired spin of the heme d iron with the nuclear magnetic moment of 19F. The axial high-spin species was converted to a new rhombic high-spin species assignable to heme b595-fluoride. The g = 2 component of this new species also gave 19F-superhyperfine splitting. These results indicate that both heme d and heme b595 can coordinate with a fluoride ion with different affinities in the fully oxidized state.

Fluoride-binding to the Escherichia coli bd-type ubiquinol oxidase studied by visible absorption and EPR spectroscopies. Publishing Authors By Initials

M Tsubaki,T Mogi,H Hori,

For similar investigative techniques: chemistry, analytical: spectrum analysis research abstracts see: investigative techniques: chemistry, analytical: spectrum analysis research

PUBMED ID PMID:

MEDLINE DATE:

Fluoride-binding to the Escherichia coli bd-type ubiquinol oxidase studied by visible absorption and EPR spectroscopies. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 126

Page Numbers: 98-103

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jul

YEAR: 1999

Fluoride-binding to the Escherichia coli bd-type ubiquinol oxidase studied by visible absorption and EPR spectroscopies. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Fluoride-binding to the Escherichia coli bd-type ubiquinol oxidase studied by visible absorption and EPR spectroscopies. Keywords Mesh Terms:

KEYWORDS: Spectrum Analysis

MESH TERMS: methods

Chemical & Substance for Abstract: Fluoride-binding to the Escherichia coli bd-type ubiquinol oxidase studied by visible absorption and EPR spectroscopies. Information

Substance Name: cytochrome bd terminal oxidase complex,

Registry Number: EC 1.9.3.-

Grant and Affiliation Information for Fluoride-binding to the Escherichia coli bd-type ubiquinol oxidase studied by visible absorption and EPR spectroscopies.

AFFILIATION: Department of Life Science, Faculty of Science, Himeji Institute of Technology, Kamigoori-cho, Akou-gun, Hyogo, 678-1297, Japan. tsubaki@sci.himeji-tech.ac.jp

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Fluoride-binding to the Escherichia coli bd-type ubiquinol oxidase studied by visible absorption and EPR spectroscopies Related Publications

• A dibenzoylmethane derivative protects dopaminergic neurons against both oxidative stress and endoplasmic reticulum stress.
• A smart video magnifier controlled by the visibility signal of a low vision user.
• Adrenodoxin-cytochrome P450scc interaction as revealed by EPR spectroscopy: comparison with the putidaredoxin-cytochrome P450cam system.
• Compact synchrotron light source of the HSRC.
• Determination of nonylphenol ethoxylates and octylphenol ethoxylates in environmental samples using 13C-labeled surrogate compounds.
• Differences in substrate selectivities of the SPOUT superfamily of methyltransferases.
• Drug-resistance gene expression and progression of astrocytic tumors.
• Effect of combined humanPTH(1-34) and calcitonin treatment in ovariectomized rats.
• Effect of intracoronary thrombectomy on 30-day mortality in patients with acute myocardial infarction.
• Effect of molecular chirality and side chain bulkiness on angiogenesis of haloacetylcarbamoyl-2-nitroimidazole compounds.
• Effect on Outcome of an Increase of Serum Cardiac Troponin T in Patients With Healing or Healed ST-Elevation Myocardial Infarction.
• Enhancement of synaptic transmission and nociceptive behaviour in HPC-1/syntaxin 1A knockout mice following peripheral nerve injury.
• Hetero subunit interaction and RNA recognition of yeast tRNA (m7G46) methyltransferase synthesized in a wheat germ cell-free translation system.
• Hydroxyl radical probing of tRNA (Gm18) methyltransferase [TrmH]-AdoMet-artificial tRNA ternary complex.
• Influence of the signal-to-noise ratio on the accuracy of IOLMaster measurements.
• Isolation of diacylglycerol-2-aminoethylphosphonate from Tetrahymena pyriformis.
• Long-term oral administration of dipyridamole improves both cardiac and physical status in patients with mild to moderate chronic heart failure: a prospective open-randomized study.
• Modified permanent middle cerebral artery occlusion rat model aiming to reduce variability in infarct size.
• Motor evoked potential mapping and monitoring by direct brainstem stimulation. Technical note.
• Nicardipine prolonged-release implants for preventing cerebral vasospasm after subarachnoid hemorrhage: effect and outcome in the first 100 patients.
• Perioperative plasma active and total ghrelin levels are reduced in acromegaly when compared with in nonfunctioning pituitary tumours even after normalization of serum GH.
• Rho-kinase activation in endothelial cells contributes to expansion of infarction after focal cerebral ischemia.
• Right-handed snakes: convergent evolution of asymmetry for functional specialization.
• Selective peripheral denervation of the levator scapulae muscle for laterocollic cervical dystonia.
• Sequence motif-specific assignment of two [2Fe-2S] clusters in rat xanthine oxidoreductase studied by site-directed mutagenesis.
• The carboxyl-terminus directs TAF(I)48 to the nucleus and nucleolus and associates with multiple nuclear import receptors.
• Vertebrate collagenase: direct extraction from animal skin and human synovial membrane.
• Visual emotional context modulates brain potentials elicited by unattended tones.
• [Current status and problems in approval of new anticancer drugs in Japan]
• [Jervell and Lange-Nielsen syndrome]