Fluorescence tomography and magnetic resonance imaging of myocardial macrophage infiltration in infarcted myocardium in vivo.

Fluorescence tomography and magnetic resonance imaging of myocardial macrophage infiltration in infarcted myocardium in vivo. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Fluorescence tomography and magnetic resonance imaging of myocardial macrophage infiltration in infarcted myocardium in vivo. Abstract Text:

David E Sosnovik,Matthias Nahrendorf,Nikolaos Deliolanis,Mikhail Novikov,Elena Aikawa,Lee Josephson,Anthony Rosenzweig,Ralph Weissleder,Vasilis Ntziachristos,

BACKGROUND: Fluorescence imaging of the heart is currently limited to invasive ex vivo or in vitro applications. We hypothesized that the adaptation of advanced transillumination and tomographic techniques would allow noninvasive fluorescence images of the heart to be acquired in vivo and be coregistered with in vivo cardiac magnetic resonance images. METHODS AND RESULTS: The uptake of the magnetofluorescent nanoparticle CLIO-Cy5.5 by macrophages in infarcted myocardium was studied. Ligation of the left coronary artery was performed in 12 mice and sham surgery in 7. The mice were injected, 48 hours after surgery, with 3 to 20 mg of iron per kilogram of CLIO-Cy5.5. Magnetic resonance imaging and fluorescence molecular tomography were performed 48 hours later. An increase in magnetic resonance imaging contrast-to-noise ratio, indicative of myocardial probe accumulation, was seen in the anterolateral walls of the infarcted mice but not in the sham-operated mice (23.0+/-2.7 versus 5.43+/-2.4; P<0.01). Fluorescence intensity over the heart was also significantly greater in the fluorescence molecular tomography images of the infarcted mice (19.1+/-5.2 versus 5.3+/-1.4; P<0.05). The uptake of CLIO-Cy5.5 by macrophages infiltrating the infarcted myocardium was confirmed by fluorescence microscopy and immunohistochemistry. CONCLUSIONS: Noninvasive imaging of myocardial macrophage infiltration has been shown to be possible by both fluorescence tomography and magnetic resonance imaging. This could be of significant value in both the research and clinical settings. The techniques developed could also be used to image other existing fluorescent and magnetofluorescent probes and could significantly expand the role of fluorescence imaging in the heart.

Fluorescence tomography and magnetic resonance imaging of myocardial macrophage infiltration in infarcted myocardium in vivo. Publishing Authors By Initials

DE Sosnovik,M Nahrendorf,N Deliolanis,M Novikov,E Aikawa,L Josephson,A Rosenzweig,R Weissleder,V Ntziachristos,

For similar diagnosis: diagnostic techniques and procedures: diagnostic imaging: tomography: tomography, optical research abstracts see: diagnosis: diagnostic techniques and procedures: diagnostic imaging: tomography: tomography, optical research

PUBMED ID PMID:

MEDLINE DATE:

Fluorescence tomography and magnetic resonance imaging of myocardial macrophage infiltration in infarcted myocardium in vivo. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Circulation

VOLUME: 115

Page Numbers: 1384-91

Journal Abbreviation: Circulation

ISSN: 1524-4539

DAY: 5

MONTH: 03

YEAR: 2007

Fluorescence tomography and magnetic resonance imaging of myocardial macrophage infiltration in infarcted myocardium in vivo. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 147763

Fluorescence tomography and magnetic resonance imaging of myocardial macrophage infiltration in infarcted myocardium in vivo. Keywords Mesh Terms:

KEYWORDS: Tomography, Optical

MESH TERMS: methods

Chemical & Substance for Abstract: Fluorescence tomography and magnetic resonance imaging of myocardial macrophage infiltration in infarcted myocardium in vivo. Information

Substance Name: Carbocyanines

Registry Number: 0

Grant and Affiliation Information for Fluorescence tomography and magnetic resonance imaging of myocardial macrophage infiltration in infarcted myocardium in vivo.

AFFILIATION: Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. sosnovik@nmr.mgh.harvard.edu

Country: United States

AGENCY: United States NHLBI

GRANT: U01 HL080731

ACRONYM: HL

MEDLINETA: Circulation

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Fluorescence tomography and magnetic resonance imaging of myocardial macrophage infiltration in infarcted myocardium in vivo Related Publications

• A submillimeter resolution fluorescence molecular imaging system for small animal imaging.
• Fast analytical approximation for arbitrary geometries in diffuse optical tomography.
• Fluorescence tomography and magnetic resonance imaging of myocardial macrophage infiltration in infarcted myocardium in vivo.
• Free-space fluorescence molecular tomography utilizing 360 degrees geometry projections.
• Hybrid photoacoustic fluorescence molecular tomography using finite-element-based inversion.
• In-vivo Lung Cancer Imaging in Mice using 360 degrees Free-space Fluorescence Molecular Tomography.
• Inversion with early photons.
• Multispectral imaging using multiple-bandpass filters.
• Multispectral photoacoustic imaging of fluorochromes in small animals.
• Optimization of 360 degrees projection fluorescence molecular tomography.
• Polarization-sensitive optoacoustic tomography of optically diffuse tissues.
• Surface Reconstruction for Free-Space 360 degrees Fluorescence Molecular Tomography and the Effects of Animal Motion.
• Time-resolved imaging of optical coefficients through murine chest cavities.
• Visualization of pulmonary inflammation using noninvasive fluorescence molecular imaging.