Fluorescence quenching as an indicator for the exposure of tryptophyl residues in Streptomyces subtilisin inhibitor.

Fluorescence quenching as an indicator for the exposure of tryptophyl residues in Streptomyces subtilisin inhibitor. Research Abstract Details 

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Fluorescence quenching as an indicator for the exposure of tryptophyl residues in Streptomyces subtilisin inhibitor. Abstract Text:

T Komiyama,M Miwa,

Fluorescence quenching by acrylamide and trichloroethanol of two equivalent tryptophyl residues in Streptomyces Subtilisin Inhibitor (SSI) was studied in several different conformations of the protein. Fluorescence intensity and polarization degree were simultaneously observed, and the relation between fluorescence intensity and lifetime was examined. The quenching profiles showed deviation from the Stern-Volmer plots. In the denatured form, a nonstoichiometric mechanism which differs from the static and dynamic one was found to be applicable to the interpretation of quenching by acrylamide. Quenching by trichloroethanol proceeds via the nonstoichiometric process for both the native (N) and the denatured (D) form. Each quenching constant obtained from SSI in N- or D-form for acrylamide or trichloroethanol quenching was compared with the others or with that from free tryptophan, as an indicator of the relative exposure of tryptophyl residues. The quenching constant of acrylamide for the urea denatured D-form is larger than that for the acid denatured D-form. The urea denatured state has more exposed tryptophyl residues. Temperature dependence of fluorescence quenching by Cs+ was also studied and a structural fluctuation was found around Trp-86 prior to the massive unfolding of the hydrophobic core containing the residue.

Fluorescence quenching as an indicator for the exposure of tryptophyl residues in Streptomyces subtilisin inhibitor. Publishing Authors By Initials

T Komiyama,M Miwa,

For similar organic chemicals: urea research abstracts see: organic chemicals: urea research

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Fluorescence quenching as an indicator for the exposure of tryptophyl residues in Streptomyces subtilisin inhibitor. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 87

Page Numbers: 1029-36

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Apr

YEAR: 1980

Fluorescence quenching as an indicator for the exposure of tryptophyl residues in Streptomyces subtilisin inhibitor. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Fluorescence quenching as an indicator for the exposure of tryptophyl residues in Streptomyces subtilisin inhibitor. Keywords Mesh Terms:

KEYWORDS: Urea

MESH TERMS: antagonists & inhibitors

Chemical & Substance for Abstract: Fluorescence quenching as an indicator for the exposure of tryptophyl residues in Streptomyces subtilisin inhibitor. Information

Substance Name: Subtilisins

Registry Number: EC 3.4.21.-

Grant and Affiliation Information for Fluorescence quenching as an indicator for the exposure of tryptophyl residues in Streptomyces subtilisin inhibitor.

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Country: JAPAN

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MEDLINETA: J Biochem

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