Fluorescence detection of DNA by the catalytic activation of an aptamer/thrombin complex.

Fluorescence detection of DNA by the catalytic activation of an aptamer/thrombin complex. Research Abstract Details 

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Fluorescence detection of DNA by the catalytic activation of an aptamer/thrombin complex. Abstract Text:

Valeri Pavlov,Bella Shlyahovsky,Itamar Willner,

A conjugate consisting of a thrombin aptamer tethered to the thrombin, Th, with a sensing nucleic acid (1) is used for the optical detection of DNA. The thrombin/aptamer complex blocks the biocatalytic functions of Th. Hybridization of the analyte DNA (2) to the sensing nucleic acid 1 yields a rigid duplex that detaches the aptamer from Th, a process that activates the protein toward the hydrolysis of bis(p-tosyl-Gly-Pro-Arg)-R110 (3) to the rhodamine 110 fluorophore (4). The system allows the DNA sensing with a sensitivity limit of 1 x 10-8 M. The aptamer/Th conjugate is also immobilized on glass slides for the optical detection of DNA. The dissociation of the aptamer/Th complex upon hybridization and the subsequent dehybridization of the duplex and the regeneration of the catalytically inactive Th/aptamer complex duplicate machinery functions.

Fluorescence detection of DNA by the catalytic activation of an aptamer/thrombin complex. Publishing Authors By Initials

V Pavlov,B Shlyahovsky,I Willner,

For similar enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases: thrombin research abstracts see: enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases: thrombin research

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Fluorescence detection of DNA by the catalytic activation of an aptamer/thrombin complex. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 127

Page Numbers: 6522-3

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 11

MONTH: May

YEAR: 2005

Fluorescence detection of DNA by the catalytic activation of an aptamer/thrombin complex. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

Fluorescence detection of DNA by the catalytic activation of an aptamer/thrombin complex. Keywords Mesh Terms:

KEYWORDS: Thrombin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Fluorescence detection of DNA by the catalytic activation of an aptamer/thrombin complex. Information

Substance Name: Thrombin

Registry Number: EC 3.4.21.5

Grant and Affiliation Information for Fluorescence detection of DNA by the catalytic activation of an aptamer/thrombin complex.

AFFILIATION: Institute of Chemistry, The Farkas Center for Light-Induced Processes, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.

Country: United States

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MEDLINETA: J Am Chem Soc

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