Flow-through lipid nanotube arrays for structure-function studies of membrane proteins by solid-state NMR spectroscopy.

Flow-through lipid nanotube arrays for structure-function studies of membrane proteins by solid-state NMR spectroscopy. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Flow-through lipid nanotube arrays for structure-function studies of membrane proteins by solid-state NMR spectroscopy. Abstract Text:

Eduard Y Chekmenev,Peter L Gor'kov,Timothy A Cross,Ali M Alaouie,Alex I Smirnov,

A novel method for studying membrane proteins in a native lipid bilayer environment by solid-state NMR spectroscopy is described and tested. Anodic aluminum oxide (AAO) substrates with flow-through 175 nm wide and 60-mum-long nanopores were employed to form macroscopically aligned peptide-containing lipid bilayers that are fluid and highly hydrated. We demonstrate that the surfaces of both leaflets of such bilayers are fully accessible to aqueous solutes. Thus, high hydration levels as well as pH and desirable ion and/or drug concentrations could be easily maintained and modified as desired in a series of experiments with the same sample. The method allows for membrane protein NMR experiments in a broad pH range that could be extended to as low as 1 and as high as 12 units for a period of up to a few hours and temperatures as high as 70 degrees C without losing the lipid alignment or bilayers from the nanopores. We demonstrate the utility of this method by a solid-state 19.6 T (17)O NMR study of reversible binding effects of mono- and divalent ions on the chemical shift properties of the Leu(10) carbonyl oxygen of transmembrane pore-forming peptide gramicidin A (gA). We further compare the (17)O shifts induced by binding metal ions to the binding of protons in the pH range from 1 to 12 and find a significant difference. This unexpected result points to a difference in mechanisms for ion and proton conduction by the gA pore. We believe that a large number of solid-state NMR-based studies, including structure-function, drug screening, proton exchange, pH, and other titration experiments, will benefit significantly from the method described here.

Flow-through lipid nanotube arrays for structure-function studies of membrane proteins by solid-state NMR spectroscopy. Publishing Authors By Initials

EY Chekmenev,PL Gor'kov,TA Cross,AM Alaouie,AI Smirnov,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Flow-through lipid nanotube arrays for structure-function studies of membrane proteins by solid-state NMR spectroscopy. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Biophysical journal

VOLUME: 91

Page Numbers: 3076-84

Journal Abbreviation:

ISSN: 0006-3495

DAY: 21

MONTH: 07

YEAR: 2006

Flow-through lipid nanotube arrays for structure-function studies of membrane proteins by solid-state NMR spectroscopy. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370626

Flow-through lipid nanotube arrays for structure-function studies of membrane proteins by solid-state NMR spectroscopy. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Flow-through lipid nanotube arrays for structure-function studies of membrane proteins by solid-state NMR spectroscopy. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Flow-through lipid nanotube arrays for structure-function studies of membrane proteins by solid-state NMR spectroscopy.

AFFILIATION: The Center for Interdisciplinary Magnetic Resonance, National High Magnetic Field Laboratory, Tallahassee, Florida 32310, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Biophys J

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Flow-through lipid nanotube arrays for structure-function studies of membrane proteins by solid-state NMR spectroscopy Related Publications

• Application of anodized aluminum in fluorescence detection of biological species.
• Electrical conductance of hydrophobic membranes or what happens below the surface.
• Factor Xa protects cultured hippocampal neurons from glutamate toxicity.
• Flow-through lipid nanotube arrays for structure-function studies of membrane proteins by solid-state NMR spectroscopy.
• High-frequency Bragg gratings in a photothermorefractive glass.
• Hydrothermally shrunk alumina nanopores and their application to DNA sensing.
• Post-processing of EPR spectra by convolution filtering: Calculation of a harmonics' series and automatic separation of fast-motion components from spin-label EPR spectra.
• Surface-assisted transient displacement charge technique. I. Photoinduced charge transfer in self-assembled monolayers.
• Surface-assisted transient displacement charge technique. II. Effect of gases on photoinduced charge transfer in self-assembled monolayers.
• Triplet energy transfer insight in coordination of unsaturated hydrocarbons by d(0) bent metallocenes (Zr, Hf).
• [Achievements and perspectives in the therapy and prevention of noncommunicable internal diseases in animals]
• [Apparatus for measurement of deflections of electrocardiograms.]
• [Determining residual amounts of streptomycin in honey and its hygienic assessment]
• [Disinfection in American and European apian foul brood]
• [Drug administration through cisternal puncture.]
• [Effect of ultraviolet irradiation and vitamin D2 on bulls fed on pressed pulp]
• [Intracavital and parietal digestion in normal and dyspeptic calves]
• [Intravital examination of bone marrow in animals]
• [Labor management, without cephalic flexion and protection of the perineum.]
• [Medical science and public health practice.]
• [Modification of the pupil in internal diseases.]
• [On ketosis in cows]
• [Pathogenic effect of helminths on the swine organism]
• [Pathogenic therapy in complications of foot-and-mouth disease]
• [Preventive treatment and its tasks]
• [Removing exudate and solutions from the cow's uterine cavity]
• [Serge? Ivanovich Smirnov]
• [The importance of controlling the spreding of suppurative bee diseases in wax plants]
• [The importance of the time of insemination of cows in prevention of sterility]
• [Volatile fatty acids in the rumen and subclinical ketosis in ruminants]