In this study we examined the effect of the specific gravity of culture medium on the frequency of hematopoietic stem cell (HSC) maintenance. We used a newly developed high-specific-gravity media. Bone marrow cells were isolated and cultured, and HSC activity was evaluated. The number of hematopoietic progenitor/stem cells was markedly higher in the medium with high specific gravity. In high-specific-gravity media, cells did not precipitate, maintenance of HSCs was increased, and there was a concomitant accumulation of beta-catenin. This novel technique for maintaining HSC populations provides an important new tool for studies in regenerative medicine.
Floating culture promotes the maintenance of hematopoietic stem cells. Publishing Authors By Initials
Floating culture promotes the maintenance of hematopoietic stem cells. Journal Published:
PUBLICATION TYPE: Journal Article
Journal: FEBS letters
VOLUME: 581
Page Numbers: 4645-50
Journal Abbreviation: FEBS Lett.
ISSN: 0014-5793
DAY: 7
MONTH: 09
YEAR: 2007
Floating culture promotes the maintenance of hematopoietic stem cells. Information
Number of References:
LANGUAGE: eng
NlmUniqueID: 155157
Floating culture promotes the maintenance of hematopoietic stem cells. Keywords Mesh Terms:
KEYWORDS: beta Catenin
MESH TERMS: metabolism
Chemical & Substance for Abstract: Floating culture promotes the maintenance of hematopoietic stem cells. Information
Substance Name: beta Catenin
Registry Number: 0
Grant and Affiliation Information for Floating culture promotes the maintenance of hematopoietic stem cells.
AFFILIATION: Department of Pediatrics, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan. kajiume@hiroshima-u.ac.jp
Country: Netherlands
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MEDLINETA: FEBS Lett
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