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Floating culture promotes the maintenance of hematopoietic stem cells.

Floating culture promotes the maintenance of hematopoietic stem cells. Research Abstract Details 

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  • Floating culture promotes the maintenance of hematopoietic stem cells. Abstract Text:

    teruyuki kajiumeTeruyuki Kajiume,louis yugeLouis Yuge,yumi kawaharaYumi Kawahara,reiko yoshimotoReiko Yoshimoto,akira sasakiAkira Sasaki,toshinori ideToshinori Ide,makoto asashimaMakoto Asashima,katsuko kataokaKatsuko Kataoka,masao kobayashiMasao Kobayashi,

    In this study we examined the effect of the specific gravity of culture medium on the frequency of hematopoietic stem cell (HSC) maintenance. We used a newly developed high-specific-gravity media. Bone marrow cells were isolated and cultured, and HSC activity was evaluated. The number of hematopoietic progenitor/stem cells was markedly higher in the medium with high specific gravity. In high-specific-gravity media, cells did not precipitate, maintenance of HSCs was increased, and there was a concomitant accumulation of beta-catenin. This novel technique for maintaining HSC populations provides an important new tool for studies in regenerative medicine.

    Floating culture promotes the maintenance of hematopoietic stem cells. Publishing Authors By Initials

    t kajiumeT Kajiume,l yugeL Yuge,y kawaharaY Kawahara,r yoshimotoR Yoshimoto,a sasakiA Sasaki,t ideT Ide,m asashimaM Asashima,k kataokaK Kataoka,m kobayashiM Kobayashi,

    For similar proteins: armadillo domain proteins: beta catenin research abstracts see: proteins: armadillo domain proteins: beta catenin research

    PUBMED ID PMID:

    MEDLINE DATE:

    Floating culture promotes the maintenance of hematopoietic stem cells. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: FEBS letters

    VOLUME: 581

    Page Numbers: 4645-50

    Journal Abbreviation: FEBS Lett.

    ISSN: 0014-5793

    DAY: 7

    MONTH: 09

    YEAR: 2007

    Floating culture promotes the maintenance of hematopoietic stem cells. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 155157

    Floating culture promotes the maintenance of hematopoietic stem cells. Keywords Mesh Terms:

    KEYWORDS: beta Catenin

    MESH TERMS: metabolism

    Chemical & Substance for Abstract: Floating culture promotes the maintenance of hematopoietic stem cells. Information

    Substance Name: beta Catenin

    Registry Number: 0

    Grant and Affiliation Information for Floating culture promotes the maintenance of hematopoietic stem cells.

    AFFILIATION: Department of Pediatrics, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan. kajiume@hiroshima-u.ac.jp

    Country: Netherlands

    Netherlands Research PublicationNetherlands Research Publication

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    MEDLINETA: FEBS Lett

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