Flavivirus serology by Western blot analysis.

Flavivirus serology by Western blot analysis. Research Abstract Details 

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Flavivirus serology by Western blot analysis. Abstract Text:

Leopoldo F Oceguera,Peter J Patiris,Robert E Chiles,Michael P Busch,Leslie H Tobler,Carl V Hanson,

The spread of West Nile virus (WNV) across the United States into areas with endemic flavivirus activity has complicated serologic surveillance of seasonal virus activity and diagnosis of infected individuals. Here we describe preliminary results from a comparison of serologic assays for flaviviruses: the reference plaque reduction neutralization test (PRNT), enzyme immunoassay (EIA), and a Western blot (WB) in which crude viral lysates were electrophoresed and blotted onto nitrocellulose. Human and chicken sera were tested and compared by each method against WNV and St. Louis encephalitis virus (SLEV). Antibody binding to three viral proteins determined WB interpretation: non-structural protein 1 (NS1), envelope (E), and pre-membrane (prM). WB results for a group of serially collected human plasma samples from WNV seroconverting blood donors were also correlated with transcription mediated amplification (TMA) and polymerase chain reaction (RT-PCR) results. Reactivity with NS1 appeared to be the most useful differentiating marker of WNV and SLEV infection in humans and chickens. Envelope protein was highly cross-reactive and, as indicated by additional results from dengue virus (DENV)-positive human sera, is perhaps useful serologically as a flavivirus group antigen.

Flavivirus serology by Western blot analysis. Publishing Authors By Initials

LF Oceguera,PJ Patiris,RE Chiles,MP Busch,LH Tobler,CV Hanson,

For similar diagnosis: laboratory techniques and procedures: immunologic tests: serologic tests research abstracts see: diagnosis: laboratory techniques and procedures: immunologic tests: serologic tests research

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Flavivirus serology by Western blot analysis. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The American journal of tropical medicine and hygi

VOLUME: 77

Page Numbers: 159-63

Journal Abbreviation: Am. J. Trop. Med. Hyg.

ISSN: 0002-9637

DAY: 3

MONTH: Jul

YEAR: 2007

Flavivirus serology by Western blot analysis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370507

Flavivirus serology by Western blot analysis. Keywords Mesh Terms:

KEYWORDS: Serologic Tests

MESH TERMS: analysis

Chemical & Substance for Abstract: Flavivirus serology by Western blot analysis. Information

Substance Name: RNA, Viral

Registry Number: 0

Grant and Affiliation Information for Flavivirus serology by Western blot analysis.

AFFILIATION: California Department of Health Services, Viral and Rickettsial Disease Laboratory, Richmond, California 94804, USA.

Country: United States

AGENCY: United States NCPDCID

GRANT: R01-CI-000214

ACRONYM: CI

MEDLINETA: Am J Trop Med Hyg

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