Flagellin glycans from two pathovars of Pseudomonas syringae contain rhamnose in D and L configurations in different ratios and modified 4-amino-4,6-dideoxyglucose.

Flagellin glycans from two pathovars of Pseudomonas syringae contain rhamnose in D and L configurations in different ratios and modified 4-amino-4,6-dideoxyglucose. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Flagellin glycans from two pathovars of Pseudomonas syringae contain rhamnose in D and L configurations in different ratios and modified 4-amino-4,6-dideoxyglucose. Abstract Text:

Kasumi Takeuchi,Hiroshi Ono,Mitsuru Yoshida,Tadashi Ishii,Etsuko Katoh,Fumiko Taguchi,Ryuji Miki,Katsuyoshi Murata,Hanae Kaku,Yuki Ichinose,Kasumi Takeuchi,Hiroshi Ono,Mitsuru Yoshida,Tadashi Ishii,Etsuko Katoh,Fumiko Taguchi,Ryuji Miki,Katsuyoshi Murata,Hanae Kaku,Yuki Ichinose,

Flagellins from Pseudomonas syringae pv. glycinea race 4 and Pseudomonas syringae pv. tabaci 6605 have been found to be glycosylated. Glycosylation of flagellin is essential for bacterial virulence and is also involved in the determination of host specificity. Flagellin glycans from both pathovars were characterized, and common sites of glycosylation were identified on six serine residues (positions 143, 164, 176, 183, 193, and 201). The structure of the glycan at serine 201 (S201) of flagellin from each pathovar was determined by sugar composition analysis, mass spectrometry, and (1)H and (13)C nuclear magnetic resonance spectroscopy. These analyses showed that the S201 glycans from both pathovars were composed of a common unique trisaccharide consisting of two rhamnosyl (Rha) residues and one modified 4-amino-4,6-dideoxyglucosyl (Qui4N) residue, beta-D-Quip4N(3-hydroxy-1-oxobutyl)2Me-(1-->3)-alpha-L-Rhap-(1-->2)-alpha-L-Rhap. Furthermore, mass analysis suggests that the glycans on each of the six serine residues are composed of similar trisaccharide units. Determination of the enantiomeric ratio of Rha from the flagellin proteins showed that flagellin from P. syringae pv. tabaci 6605 consisted solely of L-Rha, whereas P. syringae pv. glycinea race 4 flagellin contained both L-Rha and D-Rha at a molar ratio of about 4:1. Taking these findings together with those from our previous study, we conclude that these flagellin glycan structures may be important for the virulence and host specificity of P. syringae.

Flagellin glycans from two pathovars of Pseudomonas syringae contain rhamnose in D and L configurations in different ratios and modified 4-amino-4,6-dideoxyglucose. Publishing Authors By Initials

K Takeuchi,H Ono,M Yoshida,T Ishii,E Katoh,F Taguchi,R Miki,K Murata,H Kaku,Y Ichinose,K Takeuchi,H Ono,M Yoshida,T Ishii,E Katoh,F Taguchi,R Miki,K Murata,H Kaku,Y Ichinose,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Flagellin glycans from two pathovars of Pseudomonas syringae contain rhamnose in D and L configurations in different ratios and modified 4-amino-4,6-dideoxyglucose. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of bacteriology

VOLUME: 189

Page Numbers: 6945-56

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 20

MONTH: 07

YEAR: 2007

Flagellin glycans from two pathovars of Pseudomonas syringae contain rhamnose in D and L configurations in different ratios and modified 4-amino-4,6-dideoxyglucose. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985120

Flagellin glycans from two pathovars of Pseudomonas syringae contain rhamnose in D and L configurations in different ratios and modified 4-amino-4,6-dideoxyglucose. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Flagellin glycans from two pathovars of Pseudomonas syringae contain rhamnose in D and L configurations in different ratios and modified 4-amino-4,6-dideoxyglucose. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Flagellin glycans from two pathovars of Pseudomonas syringae contain rhamnose in D and L configurations in different ratios and modified 4-amino-4,6-dideoxyglucose.

AFFILIATION: National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki 305-8602, Japan. kasumit@affrc.go.jp

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Bacteriol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Flagellin glycans from two pathovars of Pseudomonas syringae contain rhamnose in D and L configurations in different ratios and modified 4-amino-4,6-dideoxyglucose Related Publications

• A long-term survivor after aggressive treatment for tracheal adenoid cystic carcinoma: a case report.
• Administration of factor XIII B subunit increased plasma factor XIII A subunit levels in factor XIII B subunit knock-out mice.
• Baroreflex control of muscle sympathetic nerve activity: Activated muscle metaboreflex in humans: 210: 1:05 PM-1:30 PM.
• Bundle-like assemblies of cadmium hydroxide nanostrands and anionic dyes.
• Comparison of bronchodilatory properties of transdermal and inhaled long-acting beta(2)-agonists.
• Decreased expression of antioxidant enzymes and increased expression of chemokines in COPD lung.
• Developmentally regulated expression of matrix metalloproteinases during fetal rat colon morphogenesis.
• Effects of glycosylation on swimming ability and flagellar polymorphic transformation in Pseudomonas syringae pv. tabaci 6605.
• Efficacy and safety of formoterol in Japanese patients with COPD.
• Expression and release of the a and b subunits for human coagulation factor XIII in baby hamster kidney (BHK) cells.
• Flagellin glycans from two pathovars of Pseudomonas syringae contain rhamnose in D and L configurations in different ratios and modified 4-amino-4,6-dideoxyglucose.
• Foam films obtained with ionic liquid.
• High-resolution MR cisternography of the cerebellopontine angle: 2D versus 3D fast spin-echo sequences.
• IF YOU HAVE A CASE AGAINST YOU.
• Improvement of pulmonary function and dyspnea by tiotropium in COPD patients using a transdermal beta(2)-agonist.
• Increased expression of tyrosine hydroxylase and anomalous neurites in catecholaminergic neurons of ATF-2 null mice.
• Isolation of 8'-Phosphate Ester Derivatives of Amicoumacins: Structure-activity Relationship of Hydroxy Amino Acid Moiety.
• MR imaging of the enlarged endolymphatic duct and sac syndrome by use of a 3D fast asymmetric spin-echo sequence: volume and signal-intensity measurement of the endolymphatic duct and sac and area measurement of the cochlear modiolus.
• Modulation of defense signal transduction by flagellin-induced WRKY41 transcription factor in Arabidopsis thaliana.
• Molecular mechanism for pterin-mediated inactivation of tyrosine hydroxylase: formation of insoluble aggregates of tyrosine hydroxylase.
• Multiple novel transcripts for apolipoprotein(a)-related gene II generated by alternative splicing in tissue- and cell type-specific manners.
• Muscle metaboreflex-induced increases in cardiac sympathetic activity vasoconstrict the coronary vasculature.
• Nitrative stress in refractory asthma.
• ON THE ISOLATION AND IDENTIFICATION OF S-METHYLCYSTEINE FROM HUMAN URINE.
• Oxidative and nitrative stress in bronchial asthma.
• Potent induction therapy with interferon and ribavirin combination therapy does not achieve a higher sustained virological response rate in chronic hepatitis C with genotype 1b and high hepatitis C virus RNA level.
• Prevalence of COPD in primary care clinics: correlation with non-respiratory diseases.
• Regulation of human protein Z gene expression by liver-enriched transcription factor HNF-4alpha and ubiquitous factor Sp1.
• Subcellular distribution of mitochondrial ribosomal RNA in the mouse oocyte and zygote.
• [Pulmonary metastatic carcinoma of the rectum growing extensively in the bronchial lumen--a case report]