First report of invertebrate Mx: cloning, characterization and expression analysis of Mx cDNA in disk abalone (Haliotis discus discus).

First report of invertebrate Mx: cloning, characterization and expression analysis of Mx cDNA in disk abalone (Haliotis discus discus). Research Abstract Details 

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First report of invertebrate Mx: cloning, characterization and expression analysis of Mx cDNA in disk abalone (Haliotis discus discus). Abstract Text:

Mahanama De Zoysa,Hyun-Sil Kang,Young-Bo Song,Youngheun Jee,Young-Don Lee,Jehee Lee,Mahanama De Zoysa,Hyun-Sil Kang,Young-Bo Song,Youngheun Jee,Young-Don Lee,Jehee Lee,

Myxovirus resistance (Mx) protein is one of the most studied antiviral proteins. It is induced by the type I interferon system (IFN alpha/beta) in various vertebrates, but its expression has not been identified or characterized in mollusks or other multi-cellular invertebrates to date. In this study, we isolated the Mx gene from a disk abalone (Haliotis discus discus) normalized cDNA library. Mx cDNA was sequenced, cloned and compared to other known Mx proteins. The full-length 1664 bp of abalone Mx cDNA contained a 1533-bp open reading frame that codes for 511 amino acids. Within the coding sequence of abalone Mx, characteristic features were found, such as a tripartite guanosine-5'-triphosphate (GTP)-binding motif and a dynamin family signature. In addition, leucine residues in the C-terminal region displayed a special leucine domain at L(468), L(475), L(489) and L(510), suggesting that abalone Mx may have a similar oligomerization function as other leucine zipper motifs. Abalone Mx protein exhibited 44% amino acid similarity with channel catfish Mx1, rainbow trout Mx2 and Atlantic halibut Mx. Abalones were injected intramuscularly with the known IFN inducer poly I:C and RT-PCR was performed for Mx mRNA analysis. The results showed enhanced Mx expression in abalone gill and digestive tissues 24h as well as 48 h after injection of poly I:C. Mx mRNA was expressed in gill, digestive gland, mantle and foot tissues in healthy abalone, suggesting that the basal level of Mx expressed is tissue-specific. There is no known Mx protein closely related to abalone Mx according to phylogenetic analysis. Abalone Mx may have diverged from a common gene ancestor of fish and mammalian Mx proteins, since abalone Mx showed high similarity in terms of conserved tripartite GTP-binding, dynamin family signature motifs and poly I:C enhancement of Mx mRNA expression.

First report of invertebrate Mx: cloning, characterization and expression analysis of Mx cDNA in disk abalone (Haliotis discus discus). Publishing Authors By Initials

M De Zoysa,HS Kang,YB Song,Y Jee,YD Lee,J Lee,M De Zoysa,HS Kang,YB Song,Y Jee,YD Lee,J Lee,

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First report of invertebrate Mx: cloning, characterization and expression analysis of Mx cDNA in disk abalone (Haliotis discus discus). Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Fish & shellfish immunology

VOLUME: 23

Page Numbers: 86-96

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ISSN: 1050-4648

DAY: 4

MONTH: 10

YEAR: 2006

First report of invertebrate Mx: cloning, characterization and expression analysis of Mx cDNA in disk abalone (Haliotis discus discus). Information

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LANGUAGE: eng

NlmUniqueID: 9505220

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AFFILIATION: Department of Marine Biotechnology, College of Ocean Science, Cheju National University, 66 Jejudaehakno, Ara-dong, Jeju, Jeju-Do 690-756, Republic of Korea.

Country: England

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MEDLINETA: Fish Shellfish Immunol

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