Fertilization of bovine oocytes induced solely with combined laser microbeam and optical tweezers.

Fertilization of bovine oocytes induced solely with combined laser microbeam and optical tweezers. Research Abstract Details 

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Fertilization of bovine oocytes induced solely with combined laser microbeam and optical tweezers. Abstract Text:

PURPOSE: Our purpose was to show that fertilization of oocytes can be obtained solely by laser light-mediated manipulation of gametes. METHOD: A small channel was drilled into the zona pellucida of bovine oocytes using an ultraviolet (UV)-laser microbeam. Highly diluted cattle sperm were not able to fertilize the laser drilled oocytes. RESULTS: Fertilization was achieved only when three to five cattle sperm were trapped with optical tweezers and inserted directly through the laser drilled hole into the perivitelline space. After 20 hr, 3 of 79 (3.8%) oocytes revealed two pronuclei and a sperm tail within their cytoplasm. Cattle sperm are difficult to catch. Therefore, the gametes had to remain for about 20 min in room atmosphere, which might be the reason for the low fertilization results. CONCLUSIONS: The results indicate that a combined UV-laser microbeam and optical tweezers trap can be used successfully for "noncontact" microinsemination procedures.

Fertilization of bovine oocytes induced solely with combined laser microbeam and optical tweezers. Publishing Authors By Initials

For similar urogenital system: genitalia: germ cells: ovum: zona pellucida research abstracts see: urogenital system: genitalia: germ cells: ovum: zona pellucida research

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Fertilization of bovine oocytes induced solely with combined laser microbeam and optical tweezers. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of assisted reproduction and genetics

VOLUME: 13

Page Numbers: 259-65

Journal Abbreviation: J. Assist. Reprod. Genet.

ISSN: 1058-0468

DAY: 17

MONTH: Mar

YEAR: 1996

Fertilization of bovine oocytes induced solely with combined laser microbeam and optical tweezers. Information

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LANGUAGE: eng

NlmUniqueID: 9206495

Fertilization of bovine oocytes induced solely with combined laser microbeam and optical tweezers. Keywords Mesh Terms:

KEYWORDS: Zona Pellucida

MESH TERMS: metabolism

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Grant and Affiliation Information for Fertilization of bovine oocytes induced solely with combined laser microbeam and optical tweezers.

AFFILIATION: Bayerische Klonierungsforschungs-GmbH & Co. KG, Oberschleissheim, Germany.

Country: UNITED STATES

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MEDLINETA: J Assist Reprod Genet

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