FepA- and TonB-dependent bacteriophage H8: receptor binding and genomic sequence.

FepA- and TonB-dependent bacteriophage H8: receptor binding and genomic sequence. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

FepA- and TonB-dependent bacteriophage H8: receptor binding and genomic sequence. Abstract Text:

Wolfgang Rabsch,Li Ma,Graham Wiley,Fares Z Najar,Wallace Kaserer,Daniel W Schuerch,Joseph E Klebba,Bruce A Roe,Jenny A Laverde Gomez,Marcus Schallmey,Salete M C Newton,Phillip E Klebba,

H8 is derived from a collection of Salmonella enterica serotype Enteritidis bacteriophage. Its morphology and genomic structure closely resemble those of bacteriophage T5 in the family Siphoviridae. H8 infected S. enterica serotypes Enteritidis and Typhimurium and Escherichia coli by initial adsorption to the outer membrane protein FepA. Ferric enterobactin inhibited H8 binding to E. coli FepA (50% inhibition concentration, 98 nM), and other ferric catecholate receptors (Fiu, Cir, and IroN) did not participate in phage adsorption. H8 infection was TonB dependent, but exbB mutations in Salmonella or E. coli did not prevent infection; only exbB tolQ or exbB tolR double mutants were resistant to H8. Experiments with deletion and substitution mutants showed that the receptor-phage interaction first involves residues distributed over the protein's outer surface and then narrows to the same charged (R316) or aromatic (Y260) residues that participate in the binding and transport of ferric enterobactin and colicins B and D. These data rationalize the multifunctionality of FepA: toxic ligands like bacteriocins and phage penetrate the outer membrane by parasitizing residues in FepA that are adapted to the transport of the natural ligand, ferric enterobactin. DNA sequence determinations revealed the complete H8 genome of 104.4 kb. A total of 120 of its 143 predicted open reading frames (ORFS) were homologous to ORFS in T5, at a level of 84% identity and 89% similarity. As in T5, the H8 structural genes clustered on the chromosome according to their function in the phage life cycle. The T5 genome contains a large section of DNA that can be deleted and that is absent in H8: compared to T5, H8 contains a 9,000-bp deletion in the early region of its chromosome, and nine potentially unique gene products. Sequence analyses of the tail proteins of phages in the same family showed that relative to pb5 (Oad) of T5 and Hrs of BF23, the FepA-binding protein (Rbp) of H8 contains unique acidic and aromatic residues. These side chains may promote binding to basic and aromatic residues in FepA that normally function in the adsorption of ferric enterobactin. Furthermore, a predicted H8 tail protein showed extensive identity and similarity to pb2 of T5, suggesting that it also functions in pore formation through the cell envelope. The variable region of this protein contains a potential TonB box, intimating that it participates in the TonB-dependent stage of the phage infection process.

FepA- and TonB-dependent bacteriophage H8: receptor binding and genomic sequence. Publishing Authors By Initials

W Rabsch,L Ma,G Wiley,FZ Najar,W Kaserer,DW Schuerch,JE Klebba,BA Roe,JA Laverde Gomez,M Schallmey,SM Newton,PE Klebba,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus attachment research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus attachment research

PUBMED ID PMID:

MEDLINE DATE:

FepA- and TonB-dependent bacteriophage H8: receptor binding and genomic sequence. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of bacteriology

VOLUME: 189

Page Numbers: 5658-74

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 25

MONTH: 05

YEAR: 2007

FepA- and TonB-dependent bacteriophage H8: receptor binding and genomic sequence. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985120

FepA- and TonB-dependent bacteriophage H8: receptor binding and genomic sequence. Keywords Mesh Terms:

KEYWORDS: Virus Attachment

MESH TERMS: ultrastructure

Chemical & Substance for Abstract: FepA- and TonB-dependent bacteriophage H8: receptor binding and genomic sequence. Information

Substance Name: Enterobactin

Registry Number: 28384-96-5

Grant and Affiliation Information for FepA- and TonB-dependent bacteriophage H8: receptor binding and genomic sequence.

AFFILIATION: Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Norman, OK 73019, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM53836

ACRONYM: GM

MEDLINETA: J Bacteriol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

FepA- and TonB-dependent bacteriophage H8: receptor binding and genomic sequence Related Publications

• Evidence of ball-and-chain transport of ferric enterobactin through FepA.
• FepA- and TonB-dependent bacteriophage H8: receptor binding and genomic sequence.
• Insight from TonB hybrid proteins into the mechanism of iron transport through the outer membrane.
• Mechanism of maltodextrin transport through LamB.
• Three paradoxes of ferric enterobactin uptake.