Fed-batch culture automated by uses of continuously measured cell concentration and culture volume.

Fed-batch culture automated by uses of continuously measured cell concentration and culture volume. Research Abstract Details 

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Fed-batch culture automated by uses of continuously measured cell concentration and culture volume. Abstract Text:

An automated control method of fed-batch culture in which the nutrient feed rate was determined from continuously measured cell concentration and culture broth volume was developed. Theoretical background was elucidated, from which it was found that the method is unique in that it controls specific substrate consumption rate of the microorganism. The method was experimentally applied to the fed-batch cultures of recombinant Escherichia coli HB101. It was observed that the specific substrate feed rate affects not only the specific growth rate but also the growth yield. If some conditions are satisfied, this type of automated fedbatch culture can be applied widely to any microbial systems and seems especially useful when the culture medium is composed of natural complex nutrient(s) because their concentrations are very difficult to detect and control.

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Fed-batch culture automated by uses of continuously measured cell concentration and culture volume. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biotechnology and bioengineering

VOLUME: 39

Page Numbers: 550-5

Journal Abbreviation: Biotechnol. Bioeng.

ISSN: 0006-3592

DAY: 5

MONTH: Mar

YEAR: 1992

Fed-batch culture automated by uses of continuously measured cell concentration and culture volume. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Fed-batch culture automated by uses of continuously measured cell concentration and culture volume.

AFFILIATION: Laboratory of Bioreaction Engineering, Department of Food Science and Technology, Faculty of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464-01, Japan.

Country: United States

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MEDLINETA: Biotechnol Bioeng

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