Feasibility assessment of cerebrospinal fluid from HIV-1-infected children for HIV proviral DNA and monocyte chemoattractant protein 1 alleles.

Feasibility assessment of cerebrospinal fluid from HIV-1-infected children for HIV proviral DNA and monocyte chemoattractant protein 1 alleles. Research Abstract Details 

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Feasibility assessment of cerebrospinal fluid from HIV-1-infected children for HIV proviral DNA and monocyte chemoattractant protein 1 alleles. Abstract Text:

Bruce Shiramizu,Emily Lau,Alyson Tamamoto,Justin Uniatowski,David Troelstrup,

The objective of the study was to assess the feasibility of measuring human immunodeficiency virus 1 (HIV-1) proviral deoxyribonucleic acid (DNA) and associated single-nucleotide polymorphism (SNP) of monocyte chemoattractant protein 1 (MCP1) in pediatric cerebrospinal fluid (CSF). The importance of HIV DNA and MCP1 SNP has been suggested to be independently important in progression to acquired immune deficiency syndrome (AIDS) and neurocognitive impairment in adults. In children, measuring both factors in the CSF may help us understand the neuropathogenic process leading to HIV-1-associated encephalopathy (HAE). Repository specimens from 27 perinatally HIV-1-infected children with HAE were assessed for HIV DNA copy by real-time polymerase chain reaction and compared with MCP1 2578G SNP mutations measured by digesting amplified 361 bp fragments. When compared with MCP1 2578G SNP, a significant number with the mutation had high HIV DNA compared with those with wild type (p < .01), with no levels detected in HIV-1-seronegative control specimens. There were six CSF specimens with enough supernatant to measure MCP1 levels by enzyme-linked immunosorbent assay, which showed high levels in those with the MCP1 2578G mutation. This study demonstrates, for the first time, that CSF HIV DNA and MCP1 SNP can be measured and could be potential tools in future clinical studies to understand the pathogenesis of pediatric HAE.

Feasibility assessment of cerebrospinal fluid from HIV-1-infected children for HIV proviral DNA and monocyte chemoattractant protein 1 alleles. Publishing Authors By Initials

B Shiramizu,E Lau,A Tamamoto,J Uniatowski,D Troelstrup,

For similar viruses: proviruses research abstracts see: viruses: proviruses research

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Feasibility assessment of cerebrospinal fluid from HIV-1-infected children for HIV proviral DNA and monocyte chemoattractant protein 1 alleles. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of investigative medicine : the official p

VOLUME: 54

Page Numbers: 468-72

Journal Abbreviation: J. Investig. Med.

ISSN: 1081-5589

DAY: 3

MONTH: Dec

YEAR: 2006

Feasibility assessment of cerebrospinal fluid from HIV-1-infected children for HIV proviral DNA and monocyte chemoattractant protein 1 alleles. Information

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LANGUAGE: eng

NlmUniqueID: 9501229

Feasibility assessment of cerebrospinal fluid from HIV-1-infected children for HIV proviral DNA and monocyte chemoattractant protein 1 alleles. Keywords Mesh Terms:

KEYWORDS: Proviruses

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Feasibility assessment of cerebrospinal fluid from HIV-1-infected children for HIV proviral DNA and monocyte chemoattractant protein 1 alleles. Information

Substance Name: DNA, Viral

Registry Number: 0

Grant and Affiliation Information for Feasibility assessment of cerebrospinal fluid from HIV-1-infected children for HIV proviral DNA and monocyte chemoattractant protein 1 alleles.

AFFILIATION: Hawaii AIDS Clinical Research Program, University of Hawaii, Honolulu, HI. bshirami@hawaii.edu

Country: Canada

AGENCY: United States NINDS

GRANT: U54NS43049

ACRONYM: NS

MEDLINETA: J Investig Med

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