Fatty acid metabolism in adipocytes: functional analysis of fatty acid transport proteins 1 and 4.

Fatty acid metabolism in adipocytes: functional analysis of fatty acid transport proteins 1 and 4. Research Abstract Details 

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Fatty acid metabolism in adipocytes: functional analysis of fatty acid transport proteins 1 and 4. Abstract Text:

Sandra Lobo,Brian M Wiczer,Ann J Smith,Angela M Hall,David A Bernlohr,

The role of fatty acid transport protein 1 (FATP1) and FATP4 in facilitating adipocyte fatty acid metabolism was investigated using stable FATP1 or FATP4 knockdown (kd) 3T3-L1 cell lines derived from retrovirus-delivered short hairpin RNA (shRNA). Decreased expression of FATP1 or FATP4 did not affect preadipocyte differentiation or the expression of FATP1 (in FATP4 kd), FATP4 (in FATP1 kd), fatty acid translocase, acyl-coenzyme A synthetase 1, and adipocyte fatty acid binding protein but did lead to increased levels of peroxisome proliferator-activated receptor gamma and CCAAT/enhancer binding protein alpha. Both FATP1 and FATP4 kd adipocytes exhibited reduced triacylglycerol deposition and corresponding reductions in diacylglycerol and monoacylglycerol levels compared with control cells. FATP1 kd adipocytes displayed an approximately 25% reduction in basal (3)H-labeled fatty acid uptake and a complete loss of insulin-stimulated (3)H-labeled fatty acid uptake compared with control adipocytes. In contrast, FATP4 kd adipocytes as well as HEK-293 cells overexpressing FATP4 did not display any changes in fatty acid influx. FATP4 kd cells exhibited increased basal lipolysis, whereas FATP1 kd cells exhibited no change in lipolytic capacity. Consistent with reduced triacylglycerol accumulation, FATP1 and FATP4 kd adipocytes exhibited enhanced 2-deoxyglucose uptake compared with control adipocytes. These findings define unique and distinct roles for FATP1 and FATP4 in adipose fatty acid metabolism.

Fatty acid metabolism in adipocytes: functional analysis of fatty acid transport proteins 1 and 4. Publishing Authors By Initials

S Lobo,BM Wiczer,AJ Smith,AM Hall,DA Bernlohr,

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Fatty acid metabolism in adipocytes: functional analysis of fatty acid transport proteins 1 and 4. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of lipid research

VOLUME: 48

Page Numbers: 609-20

Journal Abbreviation: J. Lipid Res.

ISSN: 0022-2275

DAY: 11

MONTH: 12

YEAR: 2006

Fatty acid metabolism in adipocytes: functional analysis of fatty acid transport proteins 1 and 4. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376606

Fatty acid metabolism in adipocytes: functional analysis of fatty acid transport proteins 1 and 4. Keywords Mesh Terms:

KEYWORDS: Tritium

MESH TERMS: metabolism

Chemical & Substance for Abstract: Fatty acid metabolism in adipocytes: functional analysis of fatty acid transport proteins 1 and 4. Information

Substance Name: Coenzyme A Ligases

Registry Number: EC 6.2.1.-

Grant and Affiliation Information for Fatty acid metabolism in adipocytes: functional analysis of fatty acid transport proteins 1 and 4.

AFFILIATION: Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA.

Country: United States

AGENCY: United States NIDA

GRANT: DA-053189

ACRONYM: DA

MEDLINETA: J Lipid Res

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