FASTDXL: a generalized screen to trap disulfide-stabilized complexes for use in structural studies.

FASTDXL: a generalized screen to trap disulfide-stabilized complexes for use in structural studies. Research Abstract Details 

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FASTDXL: a generalized screen to trap disulfide-stabilized complexes for use in structural studies. Abstract Text:

Jacob E Corn,James M Berger,Jacob E Corn,James M Berger,

Structural studies of macromolecular complexes have produced extraordinary insights into a wide variety of biological processes. Unfortunately, as structural biologists pursue larger and more challenging assemblies, weakly stable and/or nonspecific interactions can become significant roadblocks to structure determination. We have developed a rapid and effective pool-based screen, termed FASTDXL (focused array screening technique for disulfide X-linking), to produce and identify disulfide-stabilized protein-nucleic acid assemblies. A significant strength of FASTDXL is that it can take advantage of prior structural knowledge about molecular interactions, but does not necessarily rely upon it. A detailed application of the approach to the difficult problem of trapping a bacterial primase-ssDNA complex is described, validating the method as a route toward obtaining diffracting crystals suitable for structure determination.

FASTDXL: a generalized screen to trap disulfide-stabilized complexes for use in structural studies. Publishing Authors By Initials

JE Corn,JM Berger,JE Corn,JM Berger,

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FASTDXL: a generalized screen to trap disulfide-stabilized complexes for use in structural studies. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Structure (London, England : 1993)

VOLUME: 15

Page Numbers: 773-80

Journal Abbreviation: Structure

ISSN: 0969-2126

DAY: 3

MONTH: Jul

YEAR: 2007

FASTDXL: a generalized screen to trap disulfide-stabilized complexes for use in structural studies. Information

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LANGUAGE: eng

NlmUniqueID: 101087697

FASTDXL: a generalized screen to trap disulfide-stabilized complexes for use in structural studies. Keywords Mesh Terms:

KEYWORDS: Spectrometry, Mass, Matrix-Assisted Lase

MESH TERMS: chemistry

Chemical & Substance for Abstract: FASTDXL: a generalized screen to trap disulfide-stabilized complexes for use in structural studies. Information

Substance Name: DNA Primase

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for FASTDXL: a generalized screen to trap disulfide-stabilized complexes for use in structural studies.

AFFILIATION: Department of Molecular and Cell Biology, University of California, Berkeley, 327B Hildebrand Hall, Berkeley, CA 94720, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM071747

ACRONYM: GM

MEDLINETA: Structure

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