Fast protein evolution and germ line expression of a Drosophila parental gene and its young retroposed paralog.

Fast protein evolution and germ line expression of a Drosophila parental gene and its young retroposed paralog. Research Abstract Details 

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Fast protein evolution and germ line expression of a Drosophila parental gene and its young retroposed paralog. Abstract Text:

Esther ,Yongsheng Bai,Mansi Motiwale,

This is the first detailed study of the evolution, phylogenetic distribution, and transcription of one young retroposed gene, CG13732, and its parental gene CG15645, whose functions are unknown. CG13732 is a recognizable retroposed copy of CG15645 retaining the signals of this process. We name the parental gene Cervantes and the retrogene Quijote. To determine when this duplication occurred and the phylogenetic distribution of Quijote, we employed polymerase chain reaction, Southern blotting, and the available information on sequenced Drosophila genomes. Interestingly, these analyses revealed that Quijote is present only in 4 species of Drosophila (Drosophila melanogaster, Drosophila simulans, Drosophila sechellia, and Drosophila mauritiana) and that retroposed copies of Cervantes have also originated in the lineages leading to Drosophila yakuba and Drosophila erecta independently in the 3 instances. We name the new retrogene in the D. yakuba lineage Rocinante and the new retrogene in the D. erecta lineage Sancho. In this work, we present data on Quijote and its parental gene Cervantes. Polymorphism analysis of the derived gene and divergence data for both parental and derived genes were used to determine that both genes likely produce functional proteins and that they are changing at a fast rate (KA/KS approximately 0.38). The negative value of H of Fay and Wu in the non-African sample reveals an excess of derived variants at high frequency. This could be explained either by positive selection in the region or by demographic effects. The comparative expression pattern shows that both genes express in the same adult tissues (male and female germ line) in D. melanogaster. Quijote is also expressed in male and female in D. simulans, D. sechellia, and D. mauritiana. We argue that the fast rate of evolution of these genes could be related to their putative germ line function and are further studying the independent recruitment of Cervantes-derived retrogenes in multiple lineages.

Fast protein evolution and germ line expression of a Drosophila parental gene and its young retroposed paralog. Publishing Authors By Initials

E ,Y Bai,M Motiwale,

For similar genetic phenomena: variation (genetics) research abstracts see: genetic phenomena: variation (genetics) research

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Fast protein evolution and germ line expression of a Drosophila parental gene and its young retroposed paralog. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular biology and evolution

VOLUME: 23

Page Numbers: 2191-202

Journal Abbreviation: Mol. Biol. Evol.

ISSN: 0737-4038

DAY: 17

MONTH: 08

YEAR: 2006

Fast protein evolution and germ line expression of a Drosophila parental gene and its young retroposed paralog. Information

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LANGUAGE: eng

NlmUniqueID: 8501455

Fast protein evolution and germ line expression of a Drosophila parental gene and its young retroposed paralog. Keywords Mesh Terms:

KEYWORDS: Variation (Genetics)

MESH TERMS: metabolism

Chemical & Substance for Abstract: Fast protein evolution and germ line expression of a Drosophila parental gene and its young retroposed paralog. Information

Substance Name: Retroelements

Registry Number: 0

Grant and Affiliation Information for Fast protein evolution and germ line expression of a Drosophila parental gene and its young retroposed paralog.

AFFILIATION: Department of Biology, University of Texas at Arlington, USA. betran@uta.edu

Country: United States

AGENCY: United States NIGMS

GRANT: GM 071813-01

ACRONYM: GM

MEDLINETA: Mol Biol Evol

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