Factors involved in the germination and inactivation of Bacillus anthracis spores in murine primary macrophages.

Factors involved in the germination and inactivation of Bacillus anthracis spores in murine primary macrophages. Research Abstract Details 

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Factors involved in the germination and inactivation of Bacillus anthracis spores in murine primary macrophages. Abstract Text:

Haijing Hu,James Emerson,Arthur I Aronson,

Since macrophages have been implicated in inhalation anthrax either for defense and/or as enablers for spore trafficking, their function has been further defined. Spores were efficiently taken up by primary mouse bone marrow-derived macrophages even in the absence of serum but a minimal amount was required for spore germination and subsequent inactivation. With 10% fetal bovine serum (FBS) virtually all of the spores germinated but when the concentration of FBS was lowered to 1.0% or less, or when 10% horse serum replaced FBS, only 50% of the spores were inactivated within 1 h with no subsequent loss. Chloramphenicol, which blocks spore outgrowth but not germination, did not inhibit spore killing in macrophages. Based on complete inhibition of germination by d-alanine plus d-histidine, it is likely that only two of the several Bacillus anthracis germination systems are involved within macrophages. d-Histidine inhibits the gerH system previously implicated in germination within macrophages. d-Alanine is likely to block the gerX system since disruption of the gerXA gene resulted in little germination within 4 h in macrophages. Macrophages provide a major line of defense against infection by efficiently sequestering spores and in the presence of minimal nutrients effectively killing those that germinate before outgrowth.

Factors involved in the germination and inactivation of Bacillus anthracis spores in murine primary macrophages. Publishing Authors By Initials

H Hu,J Emerson,AI Aronson,

For similar cells: spores: spores, bacterial research abstracts see: cells: spores: spores, bacterial research

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Factors involved in the germination and inactivation of Bacillus anthracis spores in murine primary macrophages. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: FEMS microbiology letters

VOLUME: 272

Page Numbers: 245-50

Journal Abbreviation: FEMS Microbiol. Lett.

ISSN: 0378-1097

DAY: 24

MONTH: 05

YEAR: 2007

Factors involved in the germination and inactivation of Bacillus anthracis spores in murine primary macrophages. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7705721

Factors involved in the germination and inactivation of Bacillus anthracis spores in murine primary macrophages. Keywords Mesh Terms:

KEYWORDS: Spores, Bacterial

MESH TERMS: immunology

Chemical & Substance for Abstract: Factors involved in the germination and inactivation of Bacillus anthracis spores in murine primary macrophages. Information

Substance Name: Culture Media

Registry Number: 0

Grant and Affiliation Information for Factors involved in the germination and inactivation of Bacillus anthracis spores in murine primary macrophages.

AFFILIATION: Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.

Country: England

AGENCY: United States NIAID

GRANT: AI15355

ACRONYM: AI

MEDLINETA: FEMS Microbiol Lett

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