Factor Xa inhibition by immobilized recombinant tissue factor pathway inhibitor.

Factor Xa inhibition by immobilized recombinant tissue factor pathway inhibitor. Research Abstract Details 

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Factor Xa inhibition by immobilized recombinant tissue factor pathway inhibitor. Abstract Text:

Amito Chandiwal,Fowzia Shahreen Zaman,Alan E Mast,Connie L Hall,Amito Chandiwal,Fowzia Shahreen Zaman,Alan E Mast,Connie L Hall,

The recombinant form of the endogenous anticoagulant, tissue factor pathway inhibitor (rTFPI), is a potent inhibitor of Factor Xa (FXa) and the tissue factor-factor VIIa (TF:VIIa) complex. Surface-immobilized rTFPI reduces the thrombogenicity and intimal hyperplasia associated with synthetic vascular grafts in animal models and specifically reduces fibrin deposition on collagen-impregnated Dacron grafts from native blood in an in vitro flow model. The FXa inhibitory capacity of rTFPI in the bulk phase has been demonstrated in static systems and immobilized rTFPI reduces fibrin deposition in whole blood in vitro and animal studies; however, the specific mode of this anticoagulation has not been studied. Therefore, a comparison was made between the FXa binding capacity of two forms of immobilized rTFPI, i.e., passively adsorbed and covalently bound. The rTFPI-coated surfaces were evaluated using a parallel-plate flow reactor and comparing the amount of FXa exiting the flow chamber after exposure to an rTFPI-coated versus an uncoated plate. The results demonstrate that adsorbed rTFPI exhibits increased binding capacity (1.5-3.6 times) the expected stoichiometry via interactions with the C-terminus, whereas covalently-bound rTFPI interacts with FXa in a 1:1 stoichiometry. Thus, the results imply that specific FXa inhibition is a key component of the anticoagulant effect of rTFPI-coated surfaces and that passive adsorption of rTFPI to glass surfaces produces a more effective coating than covalent binding of rTFPI.

Factor Xa inhibition by immobilized recombinant tissue factor pathway inhibitor. Publishing Authors By Initials

A Chandiwal,FS Zaman,AE Mast,CL Hall,A Chandiwal,FS Zaman,AE Mast,CL Hall,

For similar proteins: recombinant proteins research abstracts see: proteins: recombinant proteins research

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MEDLINE DATE:

Factor Xa inhibition by immobilized recombinant tissue factor pathway inhibitor. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biomaterials science. Polymer edition

VOLUME: 17

Page Numbers: 1025-37

Journal Abbreviation:

ISSN: 0920-5063

DAY: 3

MONTH: 12

YEAR: 2006

Factor Xa inhibition by immobilized recombinant tissue factor pathway inhibitor. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9007393

Factor Xa inhibition by immobilized recombinant tissue factor pathway inhibitor. Keywords Mesh Terms:

KEYWORDS: Recombinant Proteins

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Factor Xa inhibition by immobilized recombinant tissue factor pathway inhibitor. Information

Substance Name: Factor Xa

Registry Number: EC 3.4.21.6

Grant and Affiliation Information for Factor Xa inhibition by immobilized recombinant tissue factor pathway inhibitor.

AFFILIATION: Department of Biomedical Engineering, The University of Memphis, Memphis, TN, USA.

Country: Netherlands

AGENCY: United States NHLBI

GRANT: HL68835

ACRONYM: HL

MEDLINETA: J Biomater Sci Polym Ed

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