Factor- and energy-free peptide synthesis promoted by aromatic tertiary amines including nucleic acid-related compounds.

Factor- and energy-free peptide synthesis promoted by aromatic tertiary amines including nucleic acid-related compounds. Research Abstract Details 

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Factor- and energy-free peptide synthesis promoted by aromatic tertiary amines including nucleic acid-related compounds. Abstract Text:

I Nitta,T Ueda,K Watanabe,

We have already reported a novel, in vitro translation system, promoted by pyridine instead of the usual protein factors and energy sources, which consists of only salt-washed ribosomes from Escherichia coli, aminoacyl-tRNA, a template RNA, Na+ and Mg2+ cations, and 40-60% pyridine [Nitta et al. (1994) J. Biochem. 115, 803-807 and the accompanying paper]. Here we show that in this system, pyridine can be replaced not only by pyridine derivatives but also by nucleobases or nucleosides, demonstrating that any compound harboring an aromatic tertiary amine within the molecule possesses such promoting activity. These compounds may serve to assist the peptide bond formation catalyzed by peptidyltransferase within ribosomes. The finding that nucleobases and nucleosides can play such a role in this reaction implies the possibility that these compounds were directly involved in the premordial translation system.

Factor- and energy-free peptide synthesis promoted by aromatic tertiary amines including nucleic acid-related compounds. Publishing Authors By Initials

I Nitta,T Ueda,K Watanabe,

For similar cells: cellular structures: intracellular space: cytoplasm: cytoplasmic structures: organelles: ribosomes research abstracts see: cells: cellular structures: intracellular space: cytoplasm: cytoplasmic structures: organelles: ribosomes research

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Factor- and energy-free peptide synthesis promoted by aromatic tertiary amines including nucleic acid-related compounds. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 118

Page Numbers: 850-4

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 1995

Factor- and energy-free peptide synthesis promoted by aromatic tertiary amines including nucleic acid-related compounds. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Factor- and energy-free peptide synthesis promoted by aromatic tertiary amines including nucleic acid-related compounds. Keywords Mesh Terms:

KEYWORDS: Ribosomes

MESH TERMS: metabolism

Chemical & Substance for Abstract: Factor- and energy-free peptide synthesis promoted by aromatic tertiary amines including nucleic acid-related compounds. Information

Substance Name: Nucleic Acids

Registry Number: 0

Grant and Affiliation Information for Factor- and energy-free peptide synthesis promoted by aromatic tertiary amines including nucleic acid-related compounds.

AFFILIATION: Department of Chemistry and Biotechnology, Faculty of Engineering, University of Tokyo.

Country: JAPAN

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MEDLINETA: J Biochem

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