Fabrication of high-permeability and high-capacity monolith for protein chromatography.

Fabrication of high-permeability and high-capacity monolith for protein chromatography. Research Abstract Details 

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Fabrication of high-permeability and high-capacity monolith for protein chromatography. Abstract Text:

Kai-Feng Du,Dong Yang,Yan Sun,

A novel approach for the fabrication of macroporous poly(glycidyl methacrylate-ethylene glycol dimethacrylate) monolith is presented. The method involved the use of sodium sulfate granules and organic solvents as co-porogens. Compared with the conventional monoliths [ML-(1-3)] using organic solvents only as a porogen, the improved monoliths [MLS-(1-3)] showed not only higher column efficiency and dynamic binding capacity (DBC) for protein (bovine serum albumin, BSA), but also higher column permeability and lower back pressure. It is considered that the superpores introduced by the solid granules played an important role for the improvement of the monolith performance. Moreover, poly(glycidyl methacrylate-diethylamine) tentacles were grafted onto the pore surface of MLS-3 monolith. This has further increased the DBC of BSA to 74.7 mg/ml, about three times higher than that of the monoliths without the grafted tentacles. This grafting does not obviously decrease the column permeability, so a new monolith of high column permeability and binding capacity has been produced for high-performance preparative protein chromatography.

Fabrication of high-permeability and high-capacity monolith for protein chromatography. Publishing Authors By Initials

KF Du,D Yang,Y Sun,

For similar proteins research abstracts see: proteins research

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Fabrication of high-permeability and high-capacity monolith for protein chromatography. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of chromatography. A

VOLUME: 1163

Page Numbers: 212-8

Journal Abbreviation:

ISSN: 0021-9673

DAY: 26

MONTH: 06

YEAR: 2007

Fabrication of high-permeability and high-capacity monolith for protein chromatography. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9318488

Fabrication of high-permeability and high-capacity monolith for protein chromatography. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: chemistry

Chemical & Substance for Abstract: Fabrication of high-permeability and high-capacity monolith for protein chromatography. Information

Substance Name: diethylamine

Registry Number: 109-89-7

Grant and Affiliation Information for Fabrication of high-permeability and high-capacity monolith for protein chromatography.

AFFILIATION: Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.

Country: Netherlands

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MEDLINETA: J Chromatogr A

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