Fabrication of conductive membrane in a polymeric electric field gradient focusing microdevice.

Fabrication of conductive membrane in a polymeric electric field gradient focusing microdevice. Research Abstract Details 

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Fabrication of conductive membrane in a polymeric electric field gradient focusing microdevice. Abstract Text:

Jikun Liu,Xuefei Sun,Paul B Farnsworth,Milton L Lee,

A novel approach to integrating a buffer ion-permeable membrane in a poly(glycidyl methacrylate-co-methyl methacrylate) micro electric field gradient focusing (muEFGF) device is described. A weir structure on which the membrane was positioned was fabricated between the separation channel and field gradient-generating channel. Before formation of the membrane, the surface of the polymeric microdevice was treated for covalent bonding of the membrane. Following surface modification, a prepolymer solution containing poly(ethylene glycol) acrylate/methacrylate and Tris-HCl buffer was loaded into the microdevice. Low-pressure nitrogen gas was then purged through the separation and field gradient-generating channels to remove the prepolymer solution from these channels. Residual prepolymer solution was retained on the weir structure due to surface tension. Finally, the premembrane was cured in place on the weir using UV radiation. Using a muEFGF device, green fluorescent protein (GFP) was concentrated 4000-fold. Separation of GFP and R-phycoerythrin, and selective elution of GFP from a protein mixture containing GFP, FITC-labeled casein, and FITC-labeled hemoglobin were also demonstrated. It was found that the membrane conductivity and presence of carboxylic acid impurities in the membrane strongly affected the behavior of the muEFGF device.

Fabrication of conductive membrane in a polymeric electric field gradient focusing microdevice. Publishing Authors By Initials

J Liu,X Sun,PB Farnsworth,ML Lee,

For similar macromolecular substances: polymers research abstracts see: macromolecular substances: polymers research

PUBMED ID PMID:

MEDLINE DATE:

Fabrication of conductive membrane in a polymeric electric field gradient focusing microdevice. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Analytical chemistry

VOLUME: 78

Page Numbers: 4654-62

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 1

MONTH: Jul

YEAR: 2006

Fabrication of conductive membrane in a polymeric electric field gradient focusing microdevice. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370536

Fabrication of conductive membrane in a polymeric electric field gradient focusing microdevice. Keywords Mesh Terms:

KEYWORDS: Polymers

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Chemical & Substance for Abstract: Fabrication of conductive membrane in a polymeric electric field gradient focusing microdevice. Information

Substance Name: Polymers

Registry Number: 0

Grant and Affiliation Information for Fabrication of conductive membrane in a polymeric electric field gradient focusing microdevice.

AFFILIATION: Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah 84602-5700, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM064547-01A1

ACRONYM: GM

MEDLINETA: Anal Chem

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