Extraction-dependent effects of American ginseng (Panax quinquefolium) on human breast cancer cell proliferation and estrogen receptor activation.

Extraction-dependent effects of American ginseng (Panax quinquefolium) on human breast cancer cell proliferation and estrogen receptor activation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Extraction-dependent effects of American ginseng (Panax quinquefolium) on human breast cancer cell proliferation and estrogen receptor activation. Abstract Text:

Mandy L King,Stuart R Adler,Laura L Murphy,

HYPOTHESIS: Ginseng root extracts and the biologically active ginsenosides have been shown to inhibit proliferation of human cancer cell lines, including breast cancer. However, there are conflicting data that suggest that ginseng extracts (GEs) may or may not have estrogenic action, which might be contraindicated in individuals with estrogen-dependent cancers. The current study was designed to address the hypothesis that the extraction method of American ginseng (Panax quinquefolium) root will dictate its ability to produce an estrogenic response using the estrogen receptor (ER)-positive MCF-7 human breast cancer cell model. METHODS: MCF-7 cells were treated with a wide concentration range of either methanol-(alc-GE) or water-extracted (w-GE) ginseng root for 6 days. Cells were grown in media containing either normal or charcoal-stripped fetal calf serum to limit exposure to exogenous estrogen. Thus, an increase in MCF-7 cell proliferation by GE indicated potential estrogenicity. This was confirmed by blocking GE-induced MCF-7 cell proliferation with ER antagonists ICI 182,780 (1 nM) and 4-hydroxytamoxifen (0.1 microM). Furthermore, the ability of GE to bind ERalpha or ERbeta and stimulate estrogen-responsive genes was examined. RESULTS: Alc-GE, but not w-GE, was able to increase MCF-7 cell proliferation at low concentrations (5-100 microg/mL) when cells were maintained under low-estrogen conditions. The stimulatory effect of alc-GE on MCF-7 cell proliferation was blocked by the ER antagonists ICI 182,780 or 4-hydroxyta-moxifen. At higher concentrations of GE, both extracts inhibited MCF-7 and ER-negative MDA-MB-231 cell proliferation regardless of media conditions. Binding assays demonstrated that alc-GE, but not w-GE, was able to bind ERalpha and ERbeta. Alc-GE (50 microg/mL) also induced an approximate 2.5-fold increase in expression of the estrogen-responsive pS2 gene, as well as progesterone receptor (PgR) gene expression, whereas w-GE was without effect. CONCLUSION: These data indicate that low concentrations of alc-GE, but not w-GE, elicit estrogenic effects, as evidenced by increased MCF-7 cell proliferation, in a manner antagonized by ER antagonists, interactions of alc-GE with estrogen receptors, and increased expression of estrogen-responsive genes by alc-GE. Thus, discrepant results between different laboratories may be due to the type of GE being analyzed for estrogenic activity.

Extraction-dependent effects of American ginseng (Panax quinquefolium) on human breast cancer cell proliferation and estrogen receptor activation. Publishing Authors By Initials

ML King,SR Adler,LL Murphy,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Extraction-dependent effects of American ginseng (Panax quinquefolium) on human breast cancer cell proliferation and estrogen receptor activation. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Integrative cancer therapies

VOLUME: 5

Page Numbers: 236-43

Journal Abbreviation:

ISSN: 1534-7354

DAY: 3

MONTH: Sep

YEAR: 2006

Extraction-dependent effects of American ginseng (Panax quinquefolium) on human breast cancer cell proliferation and estrogen receptor activation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101128834

Extraction-dependent effects of American ginseng (Panax quinquefolium) on human breast cancer cell proliferation and estrogen receptor activation. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Extraction-dependent effects of American ginseng (Panax quinquefolium) on human breast cancer cell proliferation and estrogen receptor activation. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Extraction-dependent effects of American ginseng (Panax quinquefolium) on human breast cancer cell proliferation and estrogen receptor activation.

AFFILIATION: Department of Physiology, Southern Illinois University School of Medicine, Carbondale, Illinois 62901-6523, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Integr Cancer Ther

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Extraction-dependent effects of American ginseng Panax quinquefolium on human breast cancer cell proliferation and estrogen receptor activation Related Publications

• A graphical model approach to automated classification of protein subcellular location patterns in multi-cell images.
• Application of microarray technology in psychotropic drug trials.
• Appropriate use of funds.
• Characterization of igaB, a second immunoglobulin A1 protease gene in nontypeable Haemophilus influenzae.
• Chest physiotherapy in cystic fibrosis: improved tolerance with nasal pressure support ventilation.
• Cloning and functional expression of a human eosinophil CC chemokine receptor.
• Co-expression of interleukin-6 and human growth hormone in apparently normal prostate biopsies that ultimately progress to prostate cancer using low pH, high temperature antigen retrieval.
• Cold defect on bone scan in a vertebral body after percutaneous vertebroplasty.
• Developmental expression of EphA4-tyrosine kinase receptor in the mouse brain and spinal cord.
• Distinct characteristics of murine STAT4 activation in response to IL-12 and IFN-alpha.
• Estrus synchronisation and fertility in gilts using a synthetic progestagen (Allyl Trenbolone) and inseminated with fresh stored or frozen semen.
• Extraction-dependent effects of American ginseng (Panax quinquefolium) on human breast cancer cell proliferation and estrogen receptor activation.
• Genomic copy number changes affecting the thymidylate synthase (TYMS) gene in cancer: a model for patient classification to aid fluoropyrimidine therapy.
• Human cytomegalovirus open reading frame US28 encodes a functional beta chemokine receptor.
• Immunization of cattle against modified peptides of gonadotropin releasing hormone conjugated to carriers: Effectiveness of Freund's and alternative adjuvants.
• Long-term variations in human T lymphotropic virus (HTLV)-I and HTLV-II proviral loads and association with clinical data.
• Mapping of the mouse macrophage inflammatory protein-1 alpha receptor gene Scya3r and two related mouse beta chemokine receptor-like genes to chromosome 9.
• Obsessive-compulsive disorder in Tourette syndrome.
• Positive and negative regulation of Natural Killer cells: therapeutic implications.
• Public health ethics in action: flu vaccine and drug allocation strategies.
• Sexual orientation, sexual abuse, and HIV-risk behaviors among adolescents in the Pacific Northwest.
• Source and item memory for odors and objects in children and young adults.
• Structure and functional expression of the human macrophage inflammatory protein 1 alpha/RANTES receptor.
• Subfrontal schwannoma: a case report and literature review.
• Subtotal ear canal ablation in 18 dogs and one cat with minimal distal ear canal pathology.
• THE SAND-FLIES OF THE GAMBIA (DIPTERA: PHLEBOTOMINAE).
• The L-Type voltage-gated calcium channel Cav1.3 mediates consolidation, but not extinction, of contextually conditioned fear in mice.
• The gender insulin hypothesis: why girls are born lighter than boys, and the implications for insulin resistance.
• Trauma as a precipitant of haemorrhage in synovial cysts.
• Workflow and problem domain as information planning tools in a pediatric clinic--defining present and future information technology needs.