Extracellular glutamine is a critical modulator for regulatory volume increase in human glioma cells.

Extracellular glutamine is a critical modulator for regulatory volume increase in human glioma cells. Research Abstract Details 

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Extracellular glutamine is a critical modulator for regulatory volume increase in human glioma cells. Abstract Text:

Nola Jean Ernest,Harald Sontheimer,

Mammalian cells regulate their volume to prevent unintentional changes in intracellular signaling, cell metabolism, and DNA integrity. Intentional cell volume changes occur as cells undergo proliferation, apoptosis, or cell migration. To regulate cell volume, cells use channels and transport systems to flux osmolytes across the plasma membrane followed by the obligatory movement of water. While essentially all cells are capable of regulatory volume decrease (RVD), regulatory volume increase (RVI) mechanisms have only been reported in some cell types. In this investigation, we used human glioma cells as a model system to determine conditions necessary for RVI. When exposed to hyperosmotic conditions through the addition of 30 mosM NaCl or sucrose, D54-MG and U251 glioma cell lines and glioma cells from acute patient biopsies shrunk transiently but were able to fully recover their original cell volume within 40-70 min. This ability was highly temperature sensitive and absolutely required the presence of low millimolar concentrations of l-glutamine in the extracellular solution. Other known substrates of glutamine transporters such as methyl-amino isobutyric acid (MeAIB), alanine, and threonine were unable to support RVI. The ability of cells to undergo RVI also required the presence of Na+, K+, and Cl- and was inhibited by the NKCC inhibitor, bumetanide, consistent with the involvement of a Na+/K+/2Cl- cotransporter (NKCC). Moreover, the expression of NKCC1 was demonstrated by Western blot. We concluded that regulatory volume increase in human glioma cells occurs through the uptake of Na+, K+, and Cl- by NKCC1 and is modulated by the presence of glutamine.

Extracellular glutamine is a critical modulator for regulatory volume increase in human glioma cells. Publishing Authors By Initials

NJ Ernest,H Sontheimer,

For similar proteins: carrier proteins: membrane transport proteins: ion pumps: symporters: sodium-potassium-chloride symporters research abstracts see: proteins: carrier proteins: membrane transport proteins: ion pumps: symporters: sodium-potassium-chloride symporters research

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Extracellular glutamine is a critical modulator for regulatory volume increase in human glioma cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Brain research

VOLUME: 1144

Page Numbers: 231-8

Journal Abbreviation: Brain Res.

ISSN: 0006-8993

DAY: 30

MONTH: 01

YEAR: 2007

Extracellular glutamine is a critical modulator for regulatory volume increase in human glioma cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 45503

Extracellular glutamine is a critical modulator for regulatory volume increase in human glioma cells. Keywords Mesh Terms:

KEYWORDS: Sodium-Potassium-Chloride Symporters

MESH TERMS: metabolism

Chemical & Substance for Abstract: Extracellular glutamine is a critical modulator for regulatory volume increase in human glioma cells. Information

Substance Name: Bumetanide

Registry Number: 28395-03-1

Grant and Affiliation Information for Extracellular glutamine is a critical modulator for regulatory volume increase in human glioma cells.

AFFILIATION: Department of Neurobiology, The Civitan International Research Center, University of Alabama at Birmingham, CIRC 425, 1719 6th Avenue South, Birmingham, AL 35294, USA.

Country: Netherlands

AGENCY: United States NINDS

GRANT: R01 NS036692-08

ACRONYM: NS

MEDLINETA: Brain Res

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