Extent to which hairpin opening by the Artemis:DNA-PKcs complex can contribute to junctional diversity in V(D)J recombination.

Extent to which hairpin opening by the Artemis:DNA-PKcs complex can contribute to junctional diversity in V(D)J recombination. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Extent to which hairpin opening by the Artemis:DNA-PKcs complex can contribute to junctional diversity in V(D)J recombination. Abstract Text:

Haihui Lu,Klaus Schwarz,Michael R Lieber,Haihui Lu,Klaus Schwarz,Michael R Lieber,

V(D)J recombination events are initiated by cleavage at gene segments by the RAG1:RAG2 complex, which results in hairpin formation at the coding ends. The hairpins are opened by the Artemis:DNA-PKcs complex, and then joined via the nonhomologous DNA end joining (NHEJ) process. Here we examine the opening of the hairpinned coding ends from all of the 39 functional human V(H) elements. We find that there is some sequence-dependent variation in the efficiency and even the position of hairpin opening by Artemis:DNA-PKcs. The hairpin opening efficiency varies over a 7-fold range. The hairpin opening position varies over the region from 1 to 4 nt 3' of the hairpin tip, leading to a 2-8 nt single-stranded 3' overhang at each coding end. This information provides greater clarity on the extent to which the hairpin opening position contributes to junctional diversification in V(D)J recombination.

Extent to which hairpin opening by the Artemis:DNA-PKcs complex can contribute to junctional diversity in V(D)J recombination. Publishing Authors By Initials

H Lu,K Schwarz,MR Lieber,H Lu,K Schwarz,MR Lieber,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Extent to which hairpin opening by the Artemis:DNA-PKcs complex can contribute to junctional diversity in V(D)J recombination. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Nucleic acids research

VOLUME: 35

Page Numbers: 6917-23

Journal Abbreviation: Nucleic Acids Res.

ISSN: 1362-4962

DAY: 11

MONTH: 10

YEAR: 2007

Extent to which hairpin opening by the Artemis:DNA-PKcs complex can contribute to junctional diversity in V(D)J recombination. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 411011

Extent to which hairpin opening by the Artemis:DNA-PKcs complex can contribute to junctional diversity in V(D)J recombination. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Extent to which hairpin opening by the Artemis:DNA-PKcs complex can contribute to junctional diversity in V(D)J recombination. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Extent to which hairpin opening by the Artemis:DNA-PKcs complex can contribute to junctional diversity in V(D)J recombination.

AFFILIATION: Department of Pathology, Norris Comprehensive Cancer Center, Los Angeles, CA, USA.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Nucleic Acids Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Extent to which hairpin opening by the Artemis:DNA-PKcs complex can contribute to junctional diversity in VDJ recombination Related Publications

• A helper-dependent capsid-modified adenovirus vector expressing adeno-associated virus rep78 mediates site-specific integration of a 27-kilobase transgene cassette.
• A new group B adenovirus receptor is expressed at high levels on human stem and tumor cells.
• Baculovirus-based Vaccination Vectors Allow for Efficient Induction of Immune Responses Against Plasmodium falciparum Circumsporozoite Protein.
• Caries incidence reduction by unsupervised use of 27.5% ammonium therapy dentifrice.
• Coaxial silicon nanowires as solar cells and nanoelectronic power sources.
• Combination of tumor site-located CTL-associated antigen-4 blockade and systemic regulatory T-cell depletion induces tumor-destructive immune responses.
• Comparison of adenoviruses from species B, C, e, and f after intravenous delivery.
• DENTAL CARE OF THE PREGNANT PATIENT.
• DLPC and SAMe prevent alpha1(I) collagen mRNA up-regulation in human hepatic stellate cells, whether caused by leptin or menadione.
• DNA content/ploidy as prognostic factors in prostate cancer.
• Dorsal transfer of the brachioradialis to the flexor pollicis longus enables simultaneous powering of key pinch and forearm pronation.
• Extent to which hairpin opening by the Artemis:DNA-PKcs complex can contribute to junctional diversity in V(D)J recombination.
• High stiffness of human digital flexor tendons is suited for precise finger positional control.
• In vitro evaluation of flow divertors in an elastase-induced saccular aneurysm model in rabbit.
• InAs/InP radial nanowire heterostructures as high electron mobility devices.
• Increased efficacy and decreased systemic-effects of botulinum toxin A injection after active or passive muscle manipulation.
• Intraoperative muscle measurements reveal a relationship between contracture formation and muscle remodeling.
• Intraoperative single-site sarcomere length measurement accurately reflects whole-muscle sarcomere length in the rabbit.
• Leptin represses matrix metalloproteinase-1 gene expression in LX2 human hepatic stellate cells.
• Mechanism of R-loop formation at immunoglobulin class switch sequences.
• Metabolism of ethanol and associated hepatotoxicity.
• Nanoelectronics from the bottom up.
• New serotypes of adenoviral vectors.
• Passive muscle-tendon amplitude may not reflect skeletal muscle functional excursion.
• Relationship between muscle stress and intramuscular pressure during dynamic muscle contractions.
• Structural and regulatory roles of muscle ankyrin repeat protein family in skeletal muscle.
• Synthesis of CdS and ZnS nanowires using single-source molecular precursors.
• The mechanism of human nonhomologous DNA end joining.
• Visualization of carrier depletion in semiconducting nanowires.
• [Comparison of the inhibitory effects of atropine & 2 synthetic parasympatholytics of the postinsulin & basal gastric secretion in man.]