Extended treatment with physiologic concentrations of dietary phytochemicals results in altered gene expression, reduced growth, and apoptosis of cancer cells.

Extended treatment with physiologic concentrations of dietary phytochemicals results in altered gene expression, reduced growth, and apoptosis of cancer cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Extended treatment with physiologic concentrations of dietary phytochemicals results in altered gene expression, reduced growth, and apoptosis of cancer cells. Abstract Text:

Elena P Moiseeva,Gabriela M Almeida,George D D Jones,Margaret M Manson,Elena P Moiseeva,Gabriela M Almeida,George D D Jones,Margaret M Manson,Elena P Moiseeva,Gabriela M Almeida,George D D Jones,Margaret M Manson,

Dietary phytochemicals exhibit chemopreventive potential in vivo through persistent low-dose exposures, whereas mechanistic in vitro studies with these agents generally use a high-dose single treatment. Because the latter approach is not representative of an in vivo steady state, we investigated antitumor activity of curcumin, 3,3'-diindolylmethane (DIM), epigallocatechin gallate (EGCG), genistein, or indole-3-carbinol (I3C) in breast cancer MDA-MB-231 cells, exposed in long-term culture to low concentrations, achievable in vivo. Curcumin and EGCG increased cell doubling time. Curcumin, EGCG, and I3C inhibited clonogenic growth by 55% to 60% and induced 1.5- to 2-fold higher levels of the basal caspase-3/7 activity. No changes in expression of cell cycle-related proteins or survivin were found; however, I3C reduced epidermal growth factor receptor expression, contributing to apoptosis. Because some phytochemicals are shown to inhibit DNA and histone modification, modulation of expression by the agents in a set of genes (cadherin-11, p21Cip1, urokinase-type plasminogen activator, and interleukin-6) was compared with changes induced by inhibitors of DNA methylation or histone deacetylation. The phytochemicals modified protein and/or RNA expression of these genes, with EGCG eliciting the least and DIM the most changes in gene expression. DIM and curcumin decreased cadherin-11 and increased urokinase-type plasminogen activator levels correlated with increased cell motility. Curcumin, DIM, EGCG, and genistein reduced cell sensitivity to radiation-induced DNA damage without affecting DNA repair. This model has revealed that apoptosis and not arrest is likely to be responsible for growth inhibition. It also implicated new molecular targets and activities of the agents under conditions relevant to human exposure. [Mol Cancer Ther 2007;6(11):3071-9].

Extended treatment with physiologic concentrations of dietary phytochemicals results in altered gene expression, reduced growth, and apoptosis of cancer cells. Publishing Authors By Initials

EP Moiseeva,GM Almeida,GD Jones,MM Manson,EP Moiseeva,GM Almeida,GD Jones,MM Manson,EP Moiseeva,GM Almeida,GD Jones,MM Manson,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Extended treatment with physiologic concentrations of dietary phytochemicals results in altered gene expression, reduced growth, and apoptosis of cancer cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Molecular cancer therapeutics

VOLUME: 6

Page Numbers: 3071-9

Journal Abbreviation: Mol. Cancer Ther.

ISSN: 1535-7163

DAY: 20

MONTH: Nov

YEAR: 2007

Extended treatment with physiologic concentrations of dietary phytochemicals results in altered gene expression, reduced growth, and apoptosis of cancer cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101132535

Extended treatment with physiologic concentrations of dietary phytochemicals results in altered gene expression, reduced growth, and apoptosis of cancer cells. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Extended treatment with physiologic concentrations of dietary phytochemicals results in altered gene expression, reduced growth, and apoptosis of cancer cells. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Extended treatment with physiologic concentrations of dietary phytochemicals results in altered gene expression, reduced growth, and apoptosis of cancer cells.

AFFILIATION: Cancer Biomarkers and Prevention Group, Biocentre, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom. em9@le.ac.uk.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Mol Cancer Ther

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Extended treatment with physiologic concentrations of dietary phytochemicals results in altered gene expression, reduced growth, and apoptosis of cancer cells Related Publications

• A prospective study of inflammatory cytokines and diabetes mellitus in a multiethnic cohort of postmenopausal women.
• A psychometric analysis of psychoneurotic and psychosomatic characteristics of alcoholics.
• A psychometric analysis of psychopathic characteristics of alcoholics.
• A psychometric determination of alcoholic addiction.
• A randomized factorial trial of vitamins C and E and beta carotene in the secondary prevention of cardiovascular events in women: results from the Women's Antioxidant Cardiovascular Study.
• A simple method of assessing premenstrual syndrome in large prospective studies.
• ANAEMIA AS AN AETIOLOGICAL FACTOR IN CONVERGENCE INSUFFICIENCY.
• Allelic variants in HOX genes in cryptorchidism.
• Association between diabetes and mental disorders in two American Indian reservation communities.
• Cognitive impairment, psychiatric disorders, and problematic behaviors in a tribal nursing home.
• Conduct disorder, war zone stress, and war-related posttraumatic stress disorder symptoms in American Indian Vietnam veterans.
• Diagnostic reliability of telepsychiatry in American Indian veterans.
• Dietary intakes of flavonols and flavones and coronary heart disease in US women.
• Disparities in drug use and disorder: comparison of two American Indian reservation communities and a national sample.
• Expression of nuclear notch3 in pancreatic adenocarcinomas is associated with adverse clinical features, and correlates with the expression of STAT3 and phosphorylated Akt.
• Extended treatment with physiologic concentrations of dietary phytochemicals results in altered gene expression, reduced growth, and apoptosis of cancer cells.
• Hand evaluation following ulnar forearm perforator flap harvest: a prospective study.
• HbA(1c) measured in stored erythrocytes and mortality rate among middle-aged and older women.
• High titers of transmissible spongiform encephalopathy infectivity associated with extremely low levels of PrPSc in vivo.
• Metabolism of polycyclic compounds. 9. Metabolism of 2-naphthylamine in rat tissue slices.
• Microanalyses of enzymes and metabolites in ischemia/reperfusion-induced partial-thickness skin wounds.
• My genes made me do it? The implications of behavioural genetics for responsibility and blame.
• Predicting the physiological relevance of in vitro cancer preventive activities of phytochemicals.
• Rare gas collisions with molten metal surfaces.
• Suboptimal pharmacotherapy in a tribal nursing home.
• The biochemistry of aromatic amines. 4. O-glucosiduronic acid derivatives of 2-naphthylamine.
• The biochemistry of aromatic amines. 5. 2-Aceta-mido-6-hydroxy-5-naphthyl hydrogen sulphate and other metabolites of 2-naphthylamine and 2-aceta-midonaphthalene.
• The metabolism of 2-naphthylamine in rat tissue slices.
• The metabolites of 2-naphthylamine produced by rats and rabbits.
• Tuning a bacterial chemoreceptor with protein-membrane interactions.