Expression, purification and characterization of recombinant severe acute respiratory syndrome coronavirus non-structural protein 1.

Expression, purification and characterization of recombinant severe acute respiratory syndrome coronavirus non-structural protein 1. Research Abstract Details 

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Expression, purification and characterization of recombinant severe acute respiratory syndrome coronavirus non-structural protein 1. Abstract Text:

Kimberly Brucz,Zachary J Miknis,L Wayne Schultz,Timothy C Umland,

The coronavirus (CoV) responsible for severe acute respiratory syndrome (SARS), SARS-CoV, encodes two large polyproteins (pp1a and pp1ab) that are processed by two viral proteases to yield mature non-structural proteins (nsps). Many of these nsps have essential roles in viral replication, but several have no assigned function and possess amino acid sequences that are unique to the CoV family. One such protein is SARS-CoV nsp1, which is processed from the N-terminus of both pp1a and pp1ab. The mature SARS-CoV protein is present in cells several hours post-infection and co-localizes to the viral replication complex, but its function in the viral life cycle remains unknown. Furthermore, nsp1 sequences are highly divergent across the CoV family, and it has been suggested that this is due to nsp1 possessing a function specific to viral interactions with its host cell or acting as a host specific virulence factor. In order to initiate structural and biophysical studies of SARS-CoV nsp1, a recombinant expression system and a purification protocol have been developed, yielding milligram quantities of highly purified SARS-CoV nsp1. The purified protein was characterized using circular dichroism, size exclusion chromatography, and multi-angle light scattering.

Expression, purification and characterization of recombinant severe acute respiratory syndrome coronavirus non-structural protein 1. Publishing Authors By Initials

K Brucz,ZJ Miknis,LW Schultz,TC Umland,

For similar proteins: viral proteins: viral nonstructural proteins research abstracts see: proteins: viral proteins: viral nonstructural proteins research

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MEDLINE DATE:

Expression, purification and characterization of recombinant severe acute respiratory syndrome coronavirus non-structural protein 1. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Protein expression and purification

VOLUME: 52

Page Numbers: 249-57

Journal Abbreviation: Protein Expr. Purif.

ISSN: 1046-5928

DAY: 14

MONTH: 11

YEAR: 2006

Expression, purification and characterization of recombinant severe acute respiratory syndrome coronavirus non-structural protein 1. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9101496

Expression, purification and characterization of recombinant severe acute respiratory syndrome coronavirus non-structural protein 1. Keywords Mesh Terms:

KEYWORDS: Viral Nonstructural Proteins

MESH TERMS: genetics

Chemical & Substance for Abstract: Expression, purification and characterization of recombinant severe acute respiratory syndrome coronavirus non-structural protein 1. Information

Substance Name: Peptide Hydrolases

Registry Number: EC 3.4.-

Grant and Affiliation Information for Expression, purification and characterization of recombinant severe acute respiratory syndrome coronavirus non-structural protein 1.

AFFILIATION: Hauptman-Woodward Medical Research Institute, 700 Ellicott Street, Buffalo, NY 14203, USA.

Country: United States

AGENCY: United States NIAID

GRANT: R21AI061057

ACRONYM: AI

MEDLINETA: Protein Expr Purif

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