Expression of the WT1 gene -KTS domain isoforms suppresses the invasive ability of human lung squamous cell carcinoma cells.

Expression of the WT1 gene -KTS domain isoforms suppresses the invasive ability of human lung squamous cell carcinoma cells. Research Abstract Details 

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Expression of the WT1 gene -KTS domain isoforms suppresses the invasive ability of human lung squamous cell carcinoma cells. Abstract Text:

Shogo Moriya,Masaki Takiguchi,Naohiko Seki,

Although the WT1 gene was originally isolated as a tumor suppressor gene from Wilms' tumor, oncogenic roles for WT1 have been reported in several tumors. Here, we present new findings of high levels of WT1 expression associated with the suppression of lymph node metastasis in patients with human lung squamous cell carcinoma (SCC). We investigated the effect of down-regulated WT1 gene expression on the invasive phenotype of the SCC cell line RERF-LC-AI. Invasive ability was enhanced in WT1-specific siRNA-transfected cells, and a WT1 target gene p21Waf1/Cip1 was isolated by comprehensive gene expression analysis. As several isoforms are produced from the WT1 gene, we isolated eight major WT1 isoforms from a cDNA library and cloned each variant into an expression vector. Luciferase reporter assays revealed that p21Waf1/Cip1 expression was enhanced only by the WT1 cDNA variants that included a three-amino acid deletion (-KTS). Our results suggested that the -KTS-containing variants of WT1 are directly involved in the regulation of p21Waf1/Cip1 expression and the subsequent suppression of lymph node metastasis in human lung squamous cell carcinoma.

Expression of the WT1 gene -KTS domain isoforms suppresses the invasive ability of human lung squamous cell carcinoma cells. Publishing Authors By Initials

S Moriya,M Takiguchi,N Seki,

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Expression of the WT1 gene -KTS domain isoforms suppresses the invasive ability of human lung squamous cell carcinoma cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: International journal of oncology

VOLUME: 32

Page Numbers: 349-56

Journal Abbreviation: Int. J. Oncol.

ISSN: 1019-6439

DAY: 18

MONTH: Feb

YEAR: 2008

Expression of the WT1 gene -KTS domain isoforms suppresses the invasive ability of human lung squamous cell carcinoma cells. Information

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LANGUAGE: eng

NlmUniqueID: 9306042

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Grant and Affiliation Information for Expression of the WT1 gene -KTS domain isoforms suppresses the invasive ability of human lung squamous cell carcinoma cells.

AFFILIATION: Department of Functional Genomics, Chiba University Graduate School of Medicine, Chuo, Chiba 260-8670, Japan.

Country: Greece

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MEDLINETA: Int J Oncol

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