Expression of the focal adhesion protein PINCH in normal and alkali-injured corneas and the role of PMNs.

Expression of the focal adhesion protein PINCH in normal and alkali-injured corneas and the role of PMNs. Research Abstract Details 

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Expression of the focal adhesion protein PINCH in normal and alkali-injured corneas and the role of PMNs. Abstract Text:

PURPOSE: To evaluate the role of particularly interesting new cysteine-histidine-rich protein (PINCH) in corneal wound healing and early neovascularization and to assess the influence of granulocytes. METHODS: A standardized corneal alkali wound was inflicted under general anaesthesia to the right eye of 14 New Zealand White rabbits. Seven of the rabbits received i.v. 5 mg/kg fucoidin every 2 hours to prevent granulocytes from entering the wound area. After 36 hours, the rabbits were killed, the corneas excised, fixed in 4% formaldehyde and embedded in paraffin. The sections were double-stained with antibodies against PINCH and with haematoxylin. RESULTS: In the normal cornea and limbus, PINCH was weakly expressed in the corneal epithelium and in a wedge of the conjunctival stroma. In the wounded corneas, PINCH expression was seen in the frontline of repopulating endothelial and epithelial cells, and in active keratocytes. The vascular endothelium and the granulocytes expressed PINCH, as did the conjunctival epithelium. In the fucoidin-treated rabbits, PINCH expression was markedly reduced. The vascular endothelial cells and the few granulocytes did not express PINCH in these rabbits. CONCLUSIONS: PINCH is only slightly expressed in the normal cornea. A corneal wound induces PINCH expression in the repopulating cells, in the vascular endothelial cells of the limbus, in the limbal epithelium and in the granulocytes. Exclusion of granulocytes reduces expression of PINCH and there is no expression at all in the vascular endothelium.

Expression of the focal adhesion protein PINCH in normal and alkali-injured corneas and the role of PMNs. Publishing Authors By Initials

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Expression of the focal adhesion protein PINCH in normal and alkali-injured corneas and the role of PMNs. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Acta ophthalmologica Scandinavica

VOLUME: 85

Page Numbers: 395-400

Journal Abbreviation: Acta Ophthalmol Scand

ISSN: 1395-3907

DAY: 17

MONTH: Jun

YEAR: 2007

Expression of the focal adhesion protein PINCH in normal and alkali-injured corneas and the role of PMNs. Information

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LANGUAGE: eng

NlmUniqueID: 9507578

Expression of the focal adhesion protein PINCH in normal and alkali-injured corneas and the role of PMNs. Keywords Mesh Terms:

KEYWORDS: Zinc Fingers

MESH TERMS: toxicity

Chemical & Substance for Abstract: Expression of the focal adhesion protein PINCH in normal and alkali-injured corneas and the role of PMNs. Information

Substance Name: fucoidan

Registry Number: 9072-19-9

Grant and Affiliation Information for Expression of the focal adhesion protein PINCH in normal and alkali-injured corneas and the role of PMNs.

AFFILIATION: Department of Ophthalmology, University Hospital, Linköping, Sweden. beatrice.peebo@lio.se

Country: Denmark

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MEDLINETA: Acta Ophthalmol Scand

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