Expression of the breast cancer resistance protein (Bcrp1/Abcg2) in tissues from pregnant mice: effects of pregnancy and correlations with nuclear receptors.

Expression of the breast cancer resistance protein (Bcrp1/Abcg2) in tissues from pregnant mice: effects of pregnancy and correlations with nuclear receptors. Research Abstract Details 

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Expression of the breast cancer resistance protein (Bcrp1/Abcg2) in tissues from pregnant mice: effects of pregnancy and correlations with nuclear receptors. Abstract Text:

Honggang Wang,Xiaohui Wu,Kelly Hudkins,Andrei Mikheev,Huixia Zhang,Anshul Gupta,Jashvant D Unadkat,Qingcheng Mao,

The breast cancer resistance protein (BCRP) plays an important role in drug disposition, including limiting drug penetration across the placental barrier. Our goal was to investigate the effects of pregnancy on Bcrp1 expression in pregnant mice. We examined Bcrp1 expression in placenta, kidney, liver, and small intestine at various gestational ages. Bcrp1 protein levels peaked at gestation day (gd) 15 in placenta, at gd 10 and 15 in kidney, and at gd 15 in liver; however, Bcrp1 protein levels in small intestine did not change significantly with gestational ages. Immunohistochemistry analysis revealed that the cellular localization of Bcrp1 in placenta, kidney, liver, and small intestine was not influenced by pregnancy. Bcrp1 mRNA levels were analyzed by quantitative real-time RT-PCR. In general, the effects of pregnancy on Bcrp1 protein somewhat lagged behind the effects on Bcrp1 mRNA. To further investigate the possible roles of nuclear receptors in the regulation of the Bcrp1 gene during pregnancy, we examined mRNA levels of aryl hydrocarbon receptor (AhR), hypoxia-inducible factor 1alpha (HIF1alpha), estrogen receptor alpha (ERalpha), estrogen receptor beta (ERbeta), or progesterone receptor and compared them with those of Bcrp1. Bcrp1 mRNA was significantly correlated with mRNA of AhR, HIF1alpha, and ERbeta in placenta, with mRNA of HIF1alpha in kidney, and with mRNA of AhR and ERalpha in liver. These data suggest that Bcrp1 expression in mouse tissues can be altered by pregnancy in a gestational age-dependent manner. Such effects are likely mediated by certain nuclear receptors through a transcriptional mechanism.

Expression of the breast cancer resistance protein (Bcrp1/Abcg2) in tissues from pregnant mice: effects of pregnancy and correlations with nuclear receptors. Publishing Authors By Initials

H Wang,X Wu,K Hudkins,A Mikheev,H Zhang,A Gupta,JD Unadkat,Q Mao,

For similar proteins: receptors, cytoplasmic and nuclear: receptors, steroid: receptors, progesterone research abstracts see: proteins: receptors, cytoplasmic and nuclear: receptors, steroid: receptors, progesterone research

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Expression of the breast cancer resistance protein (Bcrp1/Abcg2) in tissues from pregnant mice: effects of pregnancy and correlations with nuclear receptors. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: American journal of physiology. Endocrinology and

VOLUME: 291

Page Numbers: E1295-304

Journal Abbreviation: Am. J. Physiol. Endocrinol. Me

ISSN: 0193-1849

DAY: 3

MONTH: Dec

YEAR: 2006

Expression of the breast cancer resistance protein (Bcrp1/Abcg2) in tissues from pregnant mice: effects of pregnancy and correlations with nuclear receptors. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901226

Expression of the breast cancer resistance protein (Bcrp1/Abcg2) in tissues from pregnant mice: effects of pregnancy and correlations with nuclear receptors. Keywords Mesh Terms:

KEYWORDS: Receptors, Progesterone

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: Expression of the breast cancer resistance protein (Bcrp1/Abcg2) in tissues from pregnant mice: effects of pregnancy and correlations with nuclear receptors. Information

Substance Name: Receptors, Progesterone

Registry Number: 0

Grant and Affiliation Information for Expression of the breast cancer resistance protein (Bcrp1/Abcg2) in tissues from pregnant mice: effects of pregnancy and correlations with nuclear receptors.

AFFILIATION: Dept. of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, WA 98195-7610, USA.

Country: United States

AGENCY: United States NICHD

GRANT: HD-044404

ACRONYM: HD

MEDLINETA: Am J Physiol Endocrinol Metab

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