Expression of Th1/Th2 cytokines in human blood after in vitro treatment with chlorpyrifos, and its metabolites, in combination with endotoxin LPS and allergen Der p1.

Expression of Th1/Th2 cytokines in human blood after in vitro treatment with chlorpyrifos, and its metabolites, in combination with endotoxin LPS and allergen Der p1. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Expression of Th1/Th2 cytokines in human blood after in vitro treatment with chlorpyrifos, and its metabolites, in combination with endotoxin LPS and allergen Der p1. Abstract Text:

Paurene Duramad,Ira B Tager,John Leikauf,Brenda Eskenazi,Nina T Holland,

Exposure to organophosphate (OP) pesticides has been associated with respiratory symptoms and may be related to asthma; however, few studies have examined the molecular basis for these associations. Asthma and allergic disorders are characterized by elevated Th2 cytokines (IL-4, IL-5, IL-13), whereas the chronic inflammatory response in asthmatic airways is maintained by Th1 cytokine IFN-gamma. The goal of this in vitro study was to examine the effects of OP chlorpyrifos (CPF), and its metabolites chlorpyrifos-oxon (CPO) and 3,5,6-trichloro-2-pyridinol (TCP), singly, and in combination with endotoxin lipopolysaccharide (LPS) or house dust mite Dermatophagoides pteronyssinus (Der p1) allergen, on expression of IFN-gamma and IL-4, Th1 and Th2 signature cytokines, respectively. Cytokine expression was measured by ELISA and flow cytometry. Human blood cultures were treated with CPF/CPO/TCP (1-1000 microg ml(-1)) and LPS (1.5-2.5 microg ml(-1)) or Der p1 (200 AU ml(-1)) and supernatants were collected at 48 h. Pesticides CPF, CPO and TCP did not induce cytokine expression in vitro, while LPS and Der p1 induced IFN-gamma and IL-4 expression, respectively. Whole blood cultures treated with low doses of CPO (1 and 10 microg ml(-1)), in combination with LPS, expressed higher levels of IFN-gamma than LPS alone (P < 0.05). While CPO increased LPS-dependent induction of IFN-gamma, CPO treatment did not alter Der p1 induction of IL-4. The interaction between CPO and LPS, which results in an increased type 1 immune response, should be investigated further, particularly since the combination of OP pesticides and endotoxin is common in rural, agricultural communities.

Expression of Th1/Th2 cytokines in human blood after in vitro treatment with chlorpyrifos, and its metabolites, in combination with endotoxin LPS and allergen Der p1. Publishing Authors By Initials

P Duramad,IB Tager,J Leikauf,B Eskenazi,NT Holland,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Expression of Th1/Th2 cytokines in human blood after in vitro treatment with chlorpyrifos, and its metabolites, in combination with endotoxin LPS and allergen Der p1. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of applied toxicology : JAT

VOLUME: 26

Page Numbers: 458-65

Journal Abbreviation:

ISSN: 0260-437X

DAY: 3

MONTH: 12

YEAR: 2007

Expression of Th1/Th2 cytokines in human blood after in vitro treatment with chlorpyrifos, and its metabolites, in combination with endotoxin LPS and allergen Der p1. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8109495

Expression of Th1/Th2 cytokines in human blood after in vitro treatment with chlorpyrifos, and its metabolites, in combination with endotoxin LPS and allergen Der p1. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Expression of Th1/Th2 cytokines in human blood after in vitro treatment with chlorpyrifos, and its metabolites, in combination with endotoxin LPS and allergen Der p1. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Expression of Th1/Th2 cytokines in human blood after in vitro treatment with chlorpyrifos, and its metabolites, in combination with endotoxin LPS and allergen Der p1.

AFFILIATION: Division of Environmental Health Sciences, School of Public Health, University of California, Berkeley, California 94720, USA.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Appl Toxicol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Expression of Th1/Th2 cytokines in human blood after in vitro treatment with chlorpyrifos, and its metabolites, in combination with endotoxin LPS and allergen Der p1 Related Publications

• 2-Methoxycanthin-6-one: A New Alkaloid from the Stem Wood of Quassia amara.
• A dinucleating ligand related to the beta-diketiminates.
• Adjunctive treatment of disseminated Mycobacterium avium complex infection with interferon alpha-2b in a patient with complete interferon-gamma receptor R1 deficiency.
• AmphiBMP2/4, an amphioxus bone morphogenetic protein closely related to Drosophila decapentaplegic and vertebrate BMP2 and BMP4: insights into evolution of dorsoventral axis specification.
• Attitudes to the use of prophylaxis for thrombo-embolism in neurosurgical patients.
• Binding affinity of alkynes and alkenes to low-coordinate iron.
• Cap gun burns in children.
• Characterization of anisotropic myocardial backscatter using spectral slope, intercept and midband fit parameters.
• Cis-regulation of the amphioxus engrailed gene: insights into evolution of a muscle-specific enhancer.
• Cytokines and other immunological biomarkers in children's environmental health studies.
• Dissociable effects of disconnecting amygdala central nucleus from the ventral tegmental area or substantia nigra on learned orienting and incentive motivation.
• Expression of Th1/Th2 cytokines in human blood after in vitro treatment with chlorpyrifos, and its metabolites, in combination with endotoxin LPS and allergen Der p1.
• Fulminant mulch pneumonitis: an emergency presentation of chronic granulomatous disease.
• Functional magnetic resonance imaging of hearing-impaired children under sedation before cochlear implantation.
• Global and local development of gray and white matter volume in normal children and adolescents.
• Insertion of a MalE beta-galactosidase fusion protein into the envelope of Escherichia coli disrupts biogenesis of outer membrane proteins and processing of inner membrane proteins.
• Learned contextual cue potentiates eating in rats.
• Magnetic resonance imaging determination of tumor grade and early response to temozolomide in a genetically engineered mouse model of glioma.
• Mechanistic insight into N=N cleavage by a low-coordinate iron(II) hydride complex.
• Mouse models of brain tumors and their applications in preclinical trials.
• Notch signaling enhances nestin expression in gliomas.
• Platelet-derived growth factor-mediated gliomagenesis and brain tumor recruitment.
• Quantitative geometric descriptions of the belt iron atoms of the iron-molybdenum cofactor of nitrogenase and synthetic iron(II) model complexes.
• Rapid viral quasispecies evolution: implications for vaccine and drug strategies.
• Reinforcer-specificity of appetitive and consummatory behavior of rats after Pavlovian conditioning with food reinforcers.
• Sex differences in the development of neuroanatomical functional connectivity underlying intelligence found using Bayesian connectivity analysis.
• Sinking velocities of phytoplankton measured on a stable density gradient by laser scanning.
• Survey of Connecticut Nurse-Midwives.
• Testicular nubbins and prosthesis insertion: is it all just in the timing?
• Winter travel assignments appeal to RN, ski bum.