Expression of recombinant human thyrotropin receptor in myeloma cells.

Expression of recombinant human thyrotropin receptor in myeloma cells. Research Abstract Details 

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Expression of recombinant human thyrotropin receptor in myeloma cells. Abstract Text:

T Matsuba,M Yamada,H Suzuki,A Kanai,O Isozaki,T Yoshida,T Tsushima,K Yasukawa,

Starting with a previously isolated cDNA for human thyrotropin receptor (TSHR), we established a transformed myeloma cell line, SP56, which expresses human TSHR on its cell surface. Binding analysis showed that SP56 bears 1.1 x 10(5) TSHR per cell with a Kd of 2.2 x 10(-10) M. Using the purified cellular membrane, we established a TSH binding inhibition immunoglobulin (TBII) assay for autoantibodies against TSHR. We compared it with the TBII assay utilizing porcine thyroid membranes expressing porcine TSHR, which has been widely used for TBII assay, by using 96 serum samples from patients with autoimmune thyroid disease and normal individuals. Our TBII assay was more sensitive than the one using porcine TSHR: of 38 sera of patients which were judged negative for autoantibodies to TSHR (TBII value below 10%) by the latter assay, 28 were positive (above 20%) in our assay. By using a perfusion culture system, we obtained as many as 3 x 10(10) SP56 cells, from which 3,450 mg protein of the membrane could be purified; this is sufficient for 15,000 assays. The results indicate that the membrane of the myeloma cell line SP56 is more suitable for use in the TBII assay than the porcine thyroid membrane, in terms of sensitivity to autoantibodies against TSHR in human sera.

Expression of recombinant human thyrotropin receptor in myeloma cells. Publishing Authors By Initials

T Matsuba,M Yamada,H Suzuki,A Kanai,O Isozaki,T Yoshida,T Tsushima,K Yasukawa,

For similar cells: cells, cultured: tumor cells, cultured research abstracts see: cells: cells, cultured: tumor cells, cultured research

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Expression of recombinant human thyrotropin receptor in myeloma cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 118

Page Numbers: 265-70

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1995

Expression of recombinant human thyrotropin receptor in myeloma cells. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Expression of recombinant human thyrotropin receptor in myeloma cells. Keywords Mesh Terms:

KEYWORDS: Tumor Cells, Cultured

MESH TERMS: genetics

Chemical & Substance for Abstract: Expression of recombinant human thyrotropin receptor in myeloma cells. Information

Substance Name: thyrotropin-binding inhibitory immunoglo

Registry Number: 0

Grant and Affiliation Information for Expression of recombinant human thyrotropin receptor in myeloma cells.

AFFILIATION: Tokyo Research Center, Tosoh Corporation, Kanagawa.

Country: JAPAN

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MEDLINETA: J Biochem

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