Expression of rat renin in mammalian cells and its purification.

Expression of rat renin in mammalian cells and its purification. Research Abstract Details 

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Expression of rat renin in mammalian cells and its purification. Abstract Text:

T Yamauchi,F Suzuki,A Takahashi,I Tsutsumi,H Hori,T Watanabe,Y Ishizuka,Y Nakamura,K Murakami,

Rat renin cDNA was transfected into COS-7 and Chinese hamster ovary (CHO) cells and expressed under the control of the Simian Virus 40 early promoter. Conditioned media of the transfected cells showed renin activity only after trypsin treatment, suggesting prorenin was secreted into the medium. From the trypsinized serum-free culture of the transfected CHO cells active renin was purified to homogeneity by a simple three-step procedure. The active renin had similar specific activity, molecular weight, Km, pH optimum, and isoelectric point compared to native renin. The amino-terminal sequence was the same as that deduced from the renin cDNA. This suggests that the recombinant rat renin is similar to kidney renin in many respects, and is easily obtained by the present procedures.

Expression of rat renin in mammalian cells and its purification. Publishing Authors By Initials

T Yamauchi,F Suzuki,A Takahashi,I Tsutsumi,H Hori,T Watanabe,Y Ishizuka,Y Nakamura,K Murakami,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection research

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MEDLINE DATE:

Expression of rat renin in mammalian cells and its purification. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Clinical and experimental hypertension. Part A, Th

VOLUME: 14

Page Numbers: 377-92

Journal Abbreviation:

ISSN: 0730-0077

DAY: 12

MONTH: 02

YEAR: 1992

Expression of rat renin in mammalian cells and its purification. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8207790

Expression of rat renin in mammalian cells and its purification. Keywords Mesh Terms:

KEYWORDS: Transfection

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Expression of rat renin in mammalian cells and its purification. Information

Substance Name: Renin

Registry Number: EC 3.4.23.15

Grant and Affiliation Information for Expression of rat renin in mammalian cells and its purification.

AFFILIATION: Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.

Country: UNITED STATES

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GRANT:

ACRONYM:

MEDLINETA: Clin Exp Hypertens A

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