[Expression of OPG and RANKL at protein level in human periodontal ligament cells and the effect of 1alpha,25(OH)(2) vitamin D(3) on the secretion of OPG protein in vitro.]

[Expression of OPG and RANKL at protein level in human periodontal ligament cells and the effect of 1alpha,25(OH)(2) vitamin D(3) on the secretion of OPG protein in vitro.] Research Abstract Details 

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[Expression of OPG and RANKL at protein level in human periodontal ligament cells and the effect of 1alpha,25(OH)(2) vitamin D(3) on the secretion of OPG protein in vitro.] Abstract Text:

Ding Zhang,Yan-qi Yang,Xiao-tong Li,Min-kui Fu,

OBJECTIVE: To study the expression of osteoprotegerin(OPG) and receptor activator nuclear factor kappa B ligand(RANKL) at protein level in human periodontal ligament cells (HPDLCs), and the effect of 1alpha,25(OH)(2) vitamin D(3) [1,25(OH)(2)vitD(3)] on the secretion of OPG protein in vitro. METHODS: HPDLCs were harvested in vitro by sequential digestion with trypsin and collagenase. The expression of RANKL in HPDLCs at protein level was tested by immunocyto-chemistry. Enzyme-linked immunoadsordent assay (ELISA) was used to detect the OPG protein which was secreted into the culture medium by HPDLCs cultured with and without 10(-8) mol/L 1alpha,25 (OH)(2)vitD(3) on the 0, 2nd, 4th, and 6th days, respectively. RESULTS: RANKL protein was detected on the membrane and plasma of HPDLCs, and OPG protein was secreted in the culture medium. The secretion of OPG protein was down-regulated by 10(-8) mol/L 1alpha,25 (OH)(2)vitD(3). CONCLUSION: HPDLCs have the bone metabolism system of OPG/RANKL, which works during the process of 1alpha,25 (OH)(2)vitD(3) inducing HPDLCs. The conclusion has laid the groundwork for the study on bone remodelling mechanisms of HPDLCs.

[Expression of OPG and RANKL at protein level in human periodontal ligament cells and the effect of 1alpha,25(OH)(2) vitamin D(3) on the secretion of OPG protein in vitro.] Publishing Authors By Initials

D Zhang,YQ Yang,XT Li,MK Fu,

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[Expression of OPG and RANKL at protein level in human periodontal ligament cells and the effect of 1alpha,25(OH)(2) vitamin D(3) on the secretion of OPG protein in vitro.] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Beijing da xue xue bao. Yi xue ban = Journal of Pe

VOLUME: 36

Page Numbers: 646-9

Journal Abbreviation: Beijing Da Xue Xue Bao

ISSN: 1671-167X

DAY: 17

MONTH: Dec

YEAR: 2004

[Expression of OPG and RANKL at protein level in human periodontal ligament cells and the effect of 1alpha,25(OH)(2) vitamin D(3) on the secretion of OPG protein in vitro.] Information

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LANGUAGE: chi

NlmUniqueID: 101125284

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AFFILIATION: Department of Orthodontics, Peking University School of Stomatology, Beijing 100081, China. dingz@ht.rol.cn.net

Country: China

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MEDLINETA: Beijing Da Xue Xue Bao

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