Expression of Nox1 in 3T3 cells increases cellular acid production but not proton conductance.

Expression of Nox1 in 3T3 cells increases cellular acid production but not proton conductance. Research Abstract Details 

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Expression of Nox1 in 3T3 cells increases cellular acid production but not proton conductance. Abstract Text:

Vincent Gaggioli,Christian Schwarzer,Horst Fischer,

The role of the NADPH oxidase homolog 1 (Nox1) in plasma membrane H(+) conductance and cellular H(+) production was investigated in 3T3 cells stably expressing Nox1 (Nox1 3T3) compared to vector-expressing control cells (mock 3T3). In whole cell patch clamp experiments both Nox1 and mock 3T3 expressed a similar H(+) conductance (Nox1 3T3, 13.2+/-8.6 pS/pF; mock 3T3, 16.6+/-13.4 pS/pF) with a number of similar characteristics (e.g., current-voltage relations, current activation kinetics, Zn(2+)-sensitivity). When the intracellular pH of cells was alkalinized with NH(4)Cl, rates of intracellular acidification were significantly higher in Nox1 3T3 compared to mock 3T3. Nox1 3T3 showed a time course of acidification that followed a double-exponential function with a fast and a slow component of, on average, tau=165 s and 1780 s, whereas mock 3T3 showed only a single slow tau of 1560 s. Expression of Nox1 also caused cells to acidify the extracellular medium at higher rates than control cells; Nox1 3T3 released 96+/-19 fmol h(-1)cell(-1) of acid equivalents compared to 19+/-12 fmol h(-1)cell(-1) in mock 3T3. These data show that expression of Nox1 results in a mechanism that has the capacity to rapidly acidify the cytosol and generate significant amounts of acid. No significant effect of Nox1 expression on the plasma membrane H(+) conductance was found.

Expression of Nox1 in 3T3 cells increases cellular acid production but not proton conductance. Publishing Authors By Initials

V Gaggioli,C Schwarzer,H Fischer,

For similar inorganic chemicals: electrolytes: ions: cations: cations, monovalent: protons research abstracts see: inorganic chemicals: electrolytes: ions: cations: cations, monovalent: protons research

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MEDLINE DATE:

Expression of Nox1 in 3T3 cells increases cellular acid production but not proton conductance. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Archives of biochemistry and biophysics

VOLUME: 459

Page Numbers: 189-96

Journal Abbreviation: Arch. Biochem. Biophys.

ISSN: 0003-9861

DAY: 6

MONTH: 12

YEAR: 2006

Expression of Nox1 in 3T3 cells increases cellular acid production but not proton conductance. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 372430

Expression of Nox1 in 3T3 cells increases cellular acid production but not proton conductance. Keywords Mesh Terms:

KEYWORDS: Protons

MESH TERMS: metabolism

Chemical & Substance for Abstract: Expression of Nox1 in 3T3 cells increases cellular acid production but not proton conductance. Information

Substance Name: NADPH oxidase 1

Registry Number: EC 1.6.99.-

Grant and Affiliation Information for Expression of Nox1 in 3T3 cells increases cellular acid production but not proton conductance.

AFFILIATION: Children's Hospital Oakland Research Institute, Oakland, CA 94609, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL071829

ACRONYM: HL

MEDLINETA: Arch Biochem Biophys

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