Expression of mouse membrane-associated prostaglandin E2 synthase-2 (mPGES-2) along the urogenital tract.

Expression of mouse membrane-associated prostaglandin E2 synthase-2 (mPGES-2) along the urogenital tract. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Expression of mouse membrane-associated prostaglandin E2 synthase-2 (mPGES-2) along the urogenital tract. Abstract Text:

Guangrui Yang,Lihong Chen,Yahua Zhang,Xiaoyan Zhang,Jing Wu,Shuo Li,Mingfen Wei,Zhiwen Zhang,Matthew D Breyer,Youfei Guan,

Prostaglandin E(2) (PGE(2)) is the most common prostanoid and has a variety of bioactivities including a crucial role in urogenital function. Multiple enzymes are involved in its biosynthesis. Among 3 PGE(2) terminal synthetic enzymes, membrane-associated PGE(2) synthase-2 (mPGES-2) is the most recently identified, and its role remains uncharacterized. In previous studies, membrane-associated PGE(2) synthase-1 (mPGES-1) and cytosolic PGE(2) synthase (cPGES) were reported to be expressed along the urogenital tracts. Here we report the genomic structure and tissue distribution of mPGES-2 in the urogenital system. Analysis of several bioinformatic databases demonstrated that mouse mPGES-2 spans 7 kb and consists of 7 exons. The mPGES-2 promoter contains multiple Sp1 sites and a GC box without a TATA box motif. Real-time quantitative PCR revealed that constitutive mPGES-2 mRNA was most abundant in the heart, brain, kidney and small intestine. In the urogenital system, mPGES-2 was highly expressed in the renal cortex, followed by the renal medulla and ovary, with lower levels in the ureter, bladder and uterus. Immunohistochemistry studies indicated that mPGES-2 was ubiquitously expressed along the nephron, with much lower levels in the glomeruli. In the ureter and bladder, mPGES-2 was mainly localized to the urothelium. In the reproductive system, mPGES-2 was restricted to the epithelial cells of the testis, epididymis, vas deferens and seminal vesicle in males, and oocytes, stroma cells and corpus luteum of the ovary and epithelial cells of the oviduct and uterus in females. This expression pattern is consistent with an important role for mPGES-2-mediated PGE(2) in urogenital function.

Expression of mouse membrane-associated prostaglandin E2 synthase-2 (mPGES-2) along the urogenital tract. Publishing Authors By Initials

G Yang,L Chen,Y Zhang,X Zhang,J Wu,S Li,M Wei,Z Zhang,MD Breyer,Y Guan,

For similar urogenital system research abstracts see: urogenital system research

PUBMED ID PMID:

MEDLINE DATE:

Expression of mouse membrane-associated prostaglandin E2 synthase-2 (mPGES-2) along the urogenital tract. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochimica et biophysica acta

VOLUME: 1761

Page Numbers: 1459-68

Journal Abbreviation: Biochim. Biophys. Acta

ISSN: 0006-3002

DAY: 8

MONTH: 09

YEAR: 2006

Expression of mouse membrane-associated prostaglandin E2 synthase-2 (mPGES-2) along the urogenital tract. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 217513

Expression of mouse membrane-associated prostaglandin E2 synthase-2 (mPGES-2) along the urogenital tract. Keywords Mesh Terms:

KEYWORDS: Urogenital System

MESH TERMS: enzymology

Chemical & Substance for Abstract: Expression of mouse membrane-associated prostaglandin E2 synthase-2 (mPGES-2) along the urogenital tract. Information

Substance Name: prostaglandin-E synthase

Registry Number: EC 5.3.99.3

Grant and Affiliation Information for Expression of mouse membrane-associated prostaglandin E2 synthase-2 (mPGES-2) along the urogenital tract.

AFFILIATION: Department of Physiology and Pathophysiology, Health Science Center, Peking University, Beijing 100083, China.

Country: Netherlands

AGENCY: United States PHS

GRANT: R01 065074-03

ACRONYM: DK

MEDLINETA: Biochim Biophys Acta

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Expression of mouse membrane-associated prostaglandin E2 synthase-2 mPGES-2 along the urogenital tract Related Publications

• A convergent synthesis of new beta-turn mimics by click chemistry.
• Cell biology. Integrins, rafts, Rac, and Rho.
• Effect of electroacupuncture on reperfusion ventricular arrhythmia in rat.
• Function of the Rho family GTPases in Ras-stimulated Raf activation.
• Measurements of TSC2 GAP Activity Toward Rheb.
• Spatial self-organization in a cyclic resource-species model.
• The plexin-B1/Rac interaction inhibits PAK activation and enhances Sema4D ligand binding.
• Variants in mitochondrial tRNAGlu, tRNAArg, and tRNAThr may influence the phenotypic manifestation of deafness-associated 12S rRNA A1555G mutation in three Han Chinese families with hearing loss.
• [Association of tumor necrosis factors-beta gene polymorphism with endometriosis in women in Guangdong Province]
• [Cloning and expression analysis of lung carcinoma related gene HLCDG1]
• [Determination of germanium in A. arborescens by spectrophotometric method]
• [Determination of organophosphorous pesticide residues in red wine by solid phase microextraction-gas chromatography]
• [Determination of partition coefficient of dissolved gases in transformer oil using phase ratio variation method and static headspace gas chromatography]
• [Diagnosis and treatment for the neck lump caused by migrating foreign body from the pharynx]
• [Effects of 2A-1-1 on the aggregation and Ca2+ influx of platelets.]
• [Effects of caspase-3 inhibitor on the neuronal apoptosis in rat cerebral cortex after ischemia-reperfusion injury]
• [Efficacy of rituximab-containing regimens on indolent B-cell lymphoma--a report of 34 cases]
• [Efficacy of rituximab-containing salvage regimens on relapsed or refractory B-cell non-Hodgkin's lymphoma]
• [Expression of cellular adhesion molecules and HLA-DR antigen on vascular endothelial cells in hemangiomas.]
• [Influence of free radical inducer on the level of oxidative stress in brain of rats with fluorosis]
• [Lecture of principles and applications of capillary electrophoresis (V) Applications of capillary electrophoresis in pharmaceutical analysis and clinical chemistry]
• [Liver X receptors mediate cholesterol efflux in mouse glomerular mesangial cells]
• [Phase I clinical trial of intravenous recombinant human lymphotoxin-alpha derivative]
• [Preliminary study on planar two-dimensional capillary electrophoresis]
• [Primary central nervous system lymphoma--a report of 32 cases with literature review]
• [Progress in the study of angiopoietin-like protein 3]
• [Rat bone mesenchymal stem cells in vitro differentiation into neuron-like cells induced by Salvis miltorrhiza for injection]
• [Risk factors for nosocomial infections after total knee replacement]
• [Study of rare earth bifunctional chelate BCPDA-Eu3+ labeling protein]
• [Three-dimensional conformal radiation therapy for malignant tumors (report of 1,024 cases)]